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验证数据展示

  • WB analysis using 10477-1-AP (same clone as 10477-1-PBS)

    WB analysis using 10477-1-AP (same clone as 10477-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10477-1-AP (CHMP2A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10477-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 10477-1-AP (same clone as 10477-1-PBS)

    IHC staining of human liver cancer using 10477-1-AP (same clone as 10477-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10477-1-AP (CHMP2A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10477-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHMP2A antibody included
  • IHC staining of human liver cancer using 10477-1-AP (same clone as 10477-1-PBS)

    IHC staining of human liver cancer using 10477-1-AP (same clone as 10477-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10477-1-AP (CHMP2A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10477-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHMP2A antibody included
  • Featured Product
  • KD/KO Validated

CHMP2A Polyclonal antibody, PBS Only

CHMP2A Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10477-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

CHMP2, chromatin modifying protein 2A, Chromatin-modifying protein 2a, hVps2 1, hVps2-1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CHMP2A Ready-To-Use IHC Kit. CHMP2A primary antibody included.

产品信息

10477-1-PBS targets CHMP2A in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0719

Product name: Recombinant human CHMP2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-220 aa of BC002502

Sequence: MDLLFGRRKTPEELLRQNQRALNRAMRELDRERQKLETQEKKIIADIKKMAKQGQMDAVRIMAKDLVRTRRYVRKFVLMRANIQAVSLKIQTLKSNNSMAQAMKGVTKAMGTMNRQLKLPQIQKIMMEFERQAEIMDMKEEMMNDAIDDAMGDEEDEEESDAVVSQVLDELGLSLTDELSNLPSTGGSLSVAAGGKKAEAAASALADADADLEERLKNLR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromatin modifying protein 2A
别名 CHMP2, chromatin modifying protein 2A, Chromatin-modifying protein 2a, hVps2 1, hVps2-1
计算分子量 25 kDa
观测分子量 32 kDa
GenBank蛋白编号BC002502
基因名称 CHMP2A
Gene ID (NCBI) 27243
RRIDAB_2079470
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43633
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHMP2A, also known as Vps2-1, belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. CHMP2A is a component of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in the degradation of surface receptor proteins and the formation of endocytic multivesicular bodies (MVBs). It has been shown that CHMP2A can interact with and regulate the function of the AAA-ATPase VPS4B (PMID: 15173323). CHMP2A is also reported to be involved in HIV budding (PMID: 21396898; 23051622).

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