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  • KD/KO Validated

CHMP2A Polyclonal antibody

CHMP2A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 10477-1-AP

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Synonyms

CHMP2, CHMP2A, chromatin modifying protein 2A, hVps2 1, VPS2, Vps2 1, VPS2A



经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CHMP2A Ready-To-Use IHC Kit. CHMP2A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10477-1-AP targets CHMP2A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA Application Description
Cited ApplicationsWB, IP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen CHMP2A fusion protein Ag0719 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromatin modifying protein 2A
Synonyms CHMP2, CHMP2A, chromatin modifying protein 2A, hVps2 1, VPS2, Vps2 1, VPS2A
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC002502
Gene Symbol CHMP2A
Gene ID (NCBI) 27243
RRIDAB_2079470
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43633
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHMP2A, also known as Vps2-1, belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. CHMP2A is a component of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in the degradation of surface receptor proteins and the formation of endocytic multivesicular bodies (MVBs). It has been shown that CHMP2A can interact with and regulate the function of the AAA-ATPase VPS4B (PMID: 15173323). CHMP2A is also reported to be involved in HIV budding (PMID: 21396898; 23051622).

实验方案

Product Specific Protocols
WB protocol for CHMP2A antibody 10477-1-APDownload protocol
IHC protocol for CHMP2A antibody 10477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nature

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Authors - Shiwei Liu
humanWB

Cell

Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes

Authors - Suresh Kumar
  • KD Validated
humanWB, IF

Nature

ESCRT-III controls nuclear envelope reformation.

Authors - Yolanda Olmos
  • KD Validated
humanWB,IF

Nature

Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.

Authors - Marina Vietri
  • KD Validated
humanWB,IF

Nat Cell Biol

Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation.

Authors - Marina Vietri
  • KD Validated
humanIF

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier