验证数据展示
经过测试的应用
| Positive IF/ICC detected in | THP-1 cells |
For other applications, we recommend the unconjugated version of this antibody, 83685-4-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83685-4 targets CHI3L1/YKL40 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | CHI3L1/YKL40 fusion protein Ag33123 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | chitinase 3-like 1 (cartilage glycoprotein-39) |
| 别名 | CHI3L1, 240691F11, CGP 39, CGP-39, Chitinase 3 like protein 1 |
| 计算分子量 | 383 aa, 43 kDa |
| 观测分子量 | 40-43 kDa |
| GenBank蛋白编号 | BC008568 |
| 基因名称 | CHI3L1 |
| Gene ID (NCBI) | 1116 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P36222 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CHI3L1/YKL40 antibody CL488-83685-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |