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CHI3L1 Polyclonal antibody

CHI3L1 Polyclonal Antibody for IF-P, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF-P, WB, ELISA

Conjugate

Unconjugated

Cat No : 30419-1-AP

Print datasheet

Synonyms

39 kDa synovial protein, ASRT7, Cartilage glycoprotein 39, CGP 39, CHI3L1, Chitinase 3 like protein 1, DKFZp686N19119, FLJ38139, GP 39, GP39, HC gp39, hCGP 39, HCGP 3P, YKL 40, YKL40, YYL 40

验证数据展示

  • WB analysis using 30419-1-AP

    WB analysis using 30419-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30419-1-AP (CHI3L1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 30419-1-AP

    IF Staining of mouse brain using 30419-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CHI3L1 antibody (30419-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHT-29 cells, HepG2 cells
Positive IF detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30419-1-AP targets CHI3L1 in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF-P, WB, ELISA
Tested Reactivity Human, mouse
Immunogen CHI3L1 fusion protein Ag33123 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chitinase 3-like 1 (cartilage glycoprotein-39)
Synonyms 39 kDa synovial protein, ASRT7, Cartilage glycoprotein 39, CGP 39, CHI3L1, Chitinase 3 like protein 1, DKFZp686N19119, FLJ38139, GP 39, GP39, HC gp39, hCGP 39, HCGP 3P, YKL 40, YKL40, YYL 40
Calculated Molecular Weight 383 aa, 43 kDa
Observed Molecular Weight40-43 kDa
GenBank Accession NumberBC008568
Gene Symbol CHI3L1
Gene ID (NCBI) 1116
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36222
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHI3L1 antibody 30419-1-APDownload protocol
IF protocol for CHI3L1 antibody 30419-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols