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验证数据展示

  • WB analysis using 21334-1-AP (same clone as 21334-1-PBS)

    WB analysis using 21334-1-AP (same clone as 21334-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21334-1-AP (CHD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 21334-1-AP (same clone as 21334-1-PBS)

    WB analysis of HEK-293 using 21334-1-AP (same clone as 21334-1-PBS)

    WB result of CHD2 antibody (21334-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 21334-1-AP (same clone as 21334-1-PBS)

    IHC staining of rat brain using 21334-1-AP (same clone as 21334-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21334-1-AP (CHD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 21334-1-AP (same clone as 21334-1-PBS)

    IHC staining of rat brain using 21334-1-AP (same clone as 21334-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21334-1-AP (CHD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 21334-1-AP (same clone as 21334-1-PBS)

    IF Staining of HeLa using 21334-1-AP (same clone as 21334-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHD2 antibody (21334-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CHD2 Polyclonal antibody, PBS Only

CHD2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21334-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ATP-dependent helicase CHD2, CHD 2, CHD-2, Chromodomain-helicase-DNA-binding protein 2, EC:3.6.4.12

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

21334-1-PBS targets CHD2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag15429

Product name: Recombinant human CHD2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-230 aa of BC020810

Sequence: MMRNKDKSQEEDSSLHSNASSHSASEEASGSDSGSQSESEQGSDPGSGHGSESNSSSESSESQSESESESAGSKSQPVLPEAKEKPASKKERIADVKKMWEEYPDVYGVRRSNRSRQEPSRFNIKEEASSGSESGSPKRRGQRQLKKQEKWKQEPSEDEQEQGTSAESEPEQKKVKARRPVPRRTVPKPRVKKQPKTQRGKRKKQDSSDEDDDDDEVPKRQTRRRAAKNV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromodomain helicase DNA binding protein 2
别名 ATP-dependent helicase CHD2, CHD 2, CHD-2, Chromodomain-helicase-DNA-binding protein 2, EC:3.6.4.12
计算分子量 1828 aa, 211 kDa
观测分子量230-250 kDa
GenBank蛋白编号BC020810
基因名称 CHD2
Gene ID (NCBI) 1106
RRIDAB_3085652
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14647
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Chromodomain helicase DNA binding protein 2 (CHD2), a member of the sucrose nonfermenting (SNF-2) protein family of epigenetic regulators, which use the energy from ATP hydrolysis to change nucleosome positioning, composition and chromatin structure(PMID: 33435571). CHD2 protein plays a critical role in embryonic development, tumor suppression and survival(PMID: 24834135). In addition, CHD2 has been proposed to prevent breast cancer initiation, and CHD2 mutations are associated with chronic lymphocytic leukemia.

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