验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A431 cells, human brain tissue, Jurkat cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20576-1-AP targets CHD1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromodomain helicase DNA binding protein 1 |
| 别名 | ATP dependent helicase CHD1, CHD 1, CHD1 |
| 计算分子量 | 197 kDa |
| 观测分子量 | 200 kDa |
| GenBank蛋白编号 | NM_001270 |
| 基因名称 | CHD1 |
| Gene ID (NCBI) | 1105 |
| RRID | AB_10694288 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14646 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CHD1 belongs to the SNF2/RAD54 helicase family. CHD1 is an aTP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. CHD1 regulates polymerase II transcription. It is required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. CHD1 regulates negatively DNA replication. It is required to maintain a specific chromatin configuration across the genome. It is associated with histone deacetylase (HDAC) activity. CHD1 is required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. It functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. It is required for maintaining open chromatin and pluripotency in embryonic stem cells. In PTEN-deficient prostate and breast cancers, CHD1 depletion profoundly and specifically suppressed cell proliferation, cell survival and tumorigenic potential. The antibody is specific to CHD1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CHD1 antibody 20576-1-AP | Download protocol |
| IP protocol for CHD1 antibody 20576-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J Chromatin remodeler CHD1 promotes XPC-to-TFIIH handover of nucleosomal UV lesions in nucleotide excision repair.
| ||
Cell Prolif MATN1-AS1 promotes glioma progression by functioning as ceRNA of miR-200b/c/429 to regulate CHD1 expression. | ||
Front Behav Neurosci Impaired Contextual Fear Extinction Learning is Associated with Aberrant Regulation of CHD-Type Chromatin Remodeling Factors. | ||
Behav Brain Res Dysregulation of select ATP-dependent chromatin remodeling factors in high trait anxiety. | ||
