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验证数据展示

  • WB analysis using 87812-3-RR

    WB analysis using 87812-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87812-3-RR (CHCHD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 87812-3-RR

    WB analysis using 87812-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87812-3-RR (CHCHD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CHCHD10 Recombinant monoclonal antibody

CHCHD10 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 87812-3-RR

产品说明书

CloneNo. 253308F4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

CoraLite® Plus 488-conjugated CHCHD10 Polyclonal antibody, C22orf16, Coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial, N27C7 4, Protein N27C7 4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, mouse heart tissue, A549 cells, LNCaP cells, HEK-293 cells, rat heart tissue, mouse liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87812-3-RR targets CHCHD10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag22598

Product name: Recombinant human CHCHD10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 82-142 aa of BC065232

Sequence: QPAVQQAPTPAAPQPLQMGPCAYEIRQFLDCSTTQSDLSLCEGFSEALKQCKYYHGLSSLP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 coiled-coil-helix-coiled-coil-helix domain containing 10
别名 CoraLite® Plus 488-conjugated CHCHD10 Polyclonal antibody, C22orf16, Coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial, N27C7 4, Protein N27C7 4
计算分子量 14 kDa
观测分子量14-18 kDa
GenBank蛋白编号BC065232
基因名称 CHCHD10
Gene ID (NCBI) 400916
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8WYQ3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHCHD10 is a mitochondrial protein that is enriched at cristae junctions in the intermembrane space and is likely involved in mitochondrial genome stability and maintenance of cristae junctions. Mutations in CHCHD10 have recently been described as a cause of frontotemporal dementia (FTD) comorbid with amyotrophic lateral sclerosis (ALS).

实验方案

Product Specific Protocols
WB protocol for CHCHD10 antibody 87812-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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