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验证数据展示

  • WB analysis using 20747-1-AP (same clone as 20747-1-PBS)

    WB analysis using 20747-1-AP (same clone as 20747-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20747-1-AP (CHAT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

  • IP experiment of rat brain using 20747-1-AP (same clone as 20747-1-PBS)

    IP experiment of rat brain using 20747-1-AP (same clone as 20747-1-PBS)

    IP result of anti-CHAT (IP:20747-1-AP, 4ug; Detection:20747-1-AP 1:500) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 20747-1-AP (same clone as 20747-1-PBS)

    IHC staining of rat brain using 20747-1-AP (same clone as 20747-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHAT antibody included
  • IHC staining of mouse brain using 20747-1-AP (same clone as 20747-1-PBS)

    IHC staining of mouse brain using 20747-1-AP (same clone as 20747-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHAT antibody included
  • IHC staining of mouse brain using 20747-1-AP (same clone as 20747-1-PBS)

    IHC staining of mouse brain using 20747-1-AP (same clone as 20747-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHAT antibody included
  • IHC staining of human lung cancer using 20747-1-AP (same clone as 20747-1-PBS)

    IHC staining of human lung cancer using 20747-1-AP (same clone as 20747-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHAT antibody included
  • IF Staining of mouse brain using 20747-1-AP (same clone as 20747-1-PBS)

    IF Staining of mouse brain using 20747-1-AP (same clone as 20747-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CHAT antibody (20747-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

  • IF Staining of Neuro-2a using 20747-1-AP (same clone as 20747-1-PBS)

    IF Staining of Neuro-2a using 20747-1-AP (same clone as 20747-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using CHAT antibody (20747-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

CHAT Polyclonal antibody, PBS Only

CHAT Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 20747-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA

别名

CHOACTase, Choline acetylase, choline acetyltransferase, Choline O-acetyltransferase, EC:2.3.1.6

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

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Planning an IHC experiment? We recommend our IHCeasy CHAT Ready-To-Use IHC Kit. CHAT primary antibody included.

产品信息

20747-1-PBS targets CHAT in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 choline acetyltransferase
别名 CHOACTase, Choline acetylase, choline acetyltransferase, Choline O-acetyltransferase, EC:2.3.1.6
计算分子量 83 kDa
观测分子量 47-50 kDa, 66-70 kDa
GenBank蛋白编号NM_020549
基因名称 CHAT
Gene ID (NCBI) 1103
RRIDAB_10898169
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP28329
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons. Human CHAT gene generates multiple mRNA splice variants (PMID: 10861222). All these variants possess start codons for translation of 70 kDa cChAT protein (isoform 1). The M variant has an additional start codon for 83 kDa cChAT protein (isoform 2), which has been found in nuclei of neuronal cells in human CNS; and the S variant has another codon for 74 kDa cChAT protein (isoform 3). Besides, CHAT includes a short exon-skipping splice variant (peripheral type CHAT) pCHAT encoding 40-55 kDa (PMID: 31040770, 29759072). This antibody can recognize both pCHAT and cCHAT.

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