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验证数据展示

  • WB analysis using 66928-1-Ig (same clone as 66928-1-PBS)

    WB analysis using 66928-1-Ig (same clone as 66928-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66928-1-Ig (CFTR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

  • WB analysis using 66928-1-Ig (same clone as 66928-1-PBS)

    WB analysis using 66928-1-Ig (same clone as 66928-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66928-1-Ig (CFTR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

  • WB analysis using 66928-1-Ig (same clone as 66928-1-PBS)

    WB analysis using 66928-1-Ig (same clone as 66928-1-PBS)

    WB result of CFTR antibody (66928-1-Ig; 1:4000; room temperature for 1.5 hours) with wild-type and CFTR knockout HEK-293T cells. This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung using 66928-1-Ig (same clone as 66928-1-PBS)

    IHC staining of human lung using 66928-1-Ig (same clone as 66928-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66928-1-Ig (CFTR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CFTR antibody included
  • IHC staining of human lung using 66928-1-Ig (same clone as 66928-1-PBS)

    IHC staining of human lung using 66928-1-Ig (same clone as 66928-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66928-1-Ig (CFTR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CFTR antibody included
  • Featured Product
  • KD/KO验证

CFTR Monoclonal antibody, PBS Only

CFTR Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66928-1-PBS

产品说明书

CloneNo. 2F4A8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, rabbit

应用

WB, IHC, ELISA

别名

Channel conductance-controlling ATPase, CFTR/MRP, cAMP-dependent chloride channel, ATP-binding cassette sub-family C member 7, ABCC7

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CFTR Ready-To-Use IHC Kit. CFTR primary antibody included.

产品信息

66928-1-PBS targets CFTR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原

CatNo: Ag27810

Product name: Recombinant human CFTR protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-80 aa of NM_000492

Sequence: MQRSPLEKASVVSKLFFSWTRPILRKGYRQRLELSDIYQIPSVDSADNLSEKLEREWDRELASKKNPKLINALRRCFFWR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
别名 Channel conductance-controlling ATPase, CFTR/MRP, cAMP-dependent chloride channel, ATP-binding cassette sub-family C member 7, ABCC7
计算分子量 168 kDa
观测分子量 150 kDa
GenBank蛋白编号NM_000492
基因名称 CFTR
Gene ID (NCBI) 1080
RRIDAB_2882254
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP13569
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CFTR is a member of the ATP-binding cassette (ABC) family of membrane transport proteins, functioning as a chloride channel responsible for ion flow across epithelial surfaces of lung, sinuses, pancreas, intestine, and liver. Mutations of CFTR cause cystic fibrosis (CF), a disorder affecting the respiratory, digestive, reproductive systems and sweat glands.

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