验证数据展示
经过测试的应用
| Positive WB detected in | Calu-3 cells, HEK-293T cells, HeLa cells, HT-29 cells, Caco-2 cells, MCF-7 cells, MOLT-4 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, A431 cells, A375 cells, A549 cells, NCI-H1299 cells, HepG2 cells |
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66928-1-Ig targets CFTR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat, rabbit |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag27810 Product name: Recombinant human CFTR protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-80 aa of NM_000492 Sequence: MQRSPLEKASVVSKLFFSWTRPILRKGYRQRLELSDIYQIPSVDSADNLSEKLEREWDRELASKKNPKLINALRRCFFWR 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) |
| 别名 | Channel conductance-controlling ATPase, CFTR/MRP, cAMP-dependent chloride channel, ATP-binding cassette sub-family C member 7, ABCC7 |
| 计算分子量 | 168 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | NM_000492 |
| 基因名称 | CFTR |
| Gene ID (NCBI) | 1080 |
| RRID | AB_2882254 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P13569 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CFTR is a member of the ATP-binding cassette (ABC) family of membrane transport proteins, functioning as a chloride channel responsible for ion flow across epithelial surfaces of lung, sinuses, pancreas, intestine, and liver. Mutations of CFTR cause cystic fibrosis (CF), a disorder affecting the respiratory, digestive, reproductive systems and sweat glands.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CFTR antibody 66928-1-Ig | Download protocol |
| WB protocol for CFTR antibody 66928-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
