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验证数据展示

  • WB analysis using 18879-1-AP

    WB analysis using 18879-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18879-1-AP (CER1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse kidney using 18879-1-AP

    IF Staining of mouse kidney using 18879-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CER1 antibody (18879-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

CER1 Polyclonal antibody

CER1 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 18879-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

CER1, Cerberus, Cerberus-related protein, DAND4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU-251 cells, mouse kidney tissue, rat kidney tissue
Positive IF-P detected inmouse kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18879-1-AP targets CER1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12926

Product name: Recombinant human CER1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-267 aa of BC069405

Sequence: MHLLLFQLLVLLPLGKTTRHQDGRQNQSSLSPVLLPRNQRELPTGNHEEAEEKPDLFVAVPHLVGTSPAGEGQRQREKMLSRFGRFWKKPEREMHPSRDSDSEPFPPGTQSLIQPIDGMKMEKSPLREEAKKFWHHFMFRKTPASQGVILPIKSHEVHWETCRTVPFSQTITHEGCEKVVVQNNLCFGKCGSVHFPGAAQHSHTSCSHCLPAKFTTMHLPVNCTELSSVIKVVMLVEECQCKVKTEHEDGHILHAGSQDSFIPGVSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cerberus 1, cysteine knot superfamily, homolog (Xenopus laevis)
别名 CER1, Cerberus, Cerberus-related protein, DAND4
计算分子量 267 aa, 30 kDa
观测分子量70 kDa
GenBank蛋白编号BC069405
基因名称 CER1
Gene ID (NCBI) 9350
RRIDAB_3085568
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95813
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CER1 is a cytokine member of the cysteine knot superfamily, characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines, together with Dan and DRM/Gremlin, represent a group of bone morphogenetic protein (BMP) antagonists that can bind directly to BMPs and inhibit their activity. CER1 can exist as homo- or heterodimers (PMID: 23717584, 9501024).

实验方案

Product Specific Protocols
IF protocol for CER1 antibody 18879-1-APDownload protocol
WB protocol for CER1 antibody 18879-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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