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验证数据展示

  • WB analysis of HL-60 using 22050-1-AP (same clone as 22050-1-PBS)

    WB analysis of HL-60 using 22050-1-AP (same clone as 22050-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 22050-1-AP (same clone as 22050-1-PBS)

    IHC staining of human breast cancer using 22050-1-AP (same clone as 22050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CEP97 antibody included
  • IP experiment of HeLa using 22050-1-AP (same clone as 22050-1-PBS)

    IP experiment of HeLa using 22050-1-AP (same clone as 22050-1-PBS)

    IP result of anti-CEP97 (IP:22050-1-AP, 4ug; Detection:22050-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

  • IF Staining of MDCK using 22050-1-AP (same clone as 22050-1-PBS)

    IF Staining of MDCK using 22050-1-AP (same clone as 22050-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CEP97 antibody (22050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

CEP97 Polyclonal antibody, PBS Only

CEP97 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 22050-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, canine

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

centrosomal protein 97kDa, Centrosomal protein of 97 kDa, Leucine-rich repeat and IQ domain-containing protein 2, LRRIQ2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CEP97 Ready-To-Use IHC Kit. CEP97 primary antibody included.

产品信息

22050-1-PBS targets CEP97 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, canine samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, canine
免疫原

CatNo: Ag16995

Product name: Recombinant human CEP97 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 506-865 aa of BC041085

Sequence: ESTEQKQSDIKKPENTQPENKETISQATSEKLPMILTQRSVALGQDKVALQKLNDAATKLQACWRGFYARNYNPQAKDVRYEIRLRRMQEHIVCLTDEIRRLRKERDEERIKKFVQEEAFRFLWNQVRSLQVWQQTVDQRLSSWHTDVPPISSTLVPSKHPLFTQSQESSCDQNADWFIASDVAPQEKSLPEFPDSGFHSSLTEQVHSLQHSLDFEKSSTEGSESSIMGNSIDTVRYGKESDLGDVSEEHGEWNKESSNNEQDNSLLEQYLTSVQQLEDADERTNFDTETRDSKLHIACFPVQLDTLSDGASVDESHGISPPLQGEISQTQENSKLNAEVQGQQPECDSTFQLLHVGVTV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 centrosomal protein 97kDa
别名 centrosomal protein 97kDa, Centrosomal protein of 97 kDa, Leucine-rich repeat and IQ domain-containing protein 2, LRRIQ2
计算分子量 865 aa, 97 kDa
观测分子量 130-140 kDa
GenBank蛋白编号BC041085
基因名称 CEP97
Gene ID (NCBI) 79598
RRIDAB_11182378
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IW35
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CEP97, also named as LRRIQ2, contains one IQ domain, eight LRR (leucine-rich) repeats and one LRRCT domain. It collaborates with CEP110, being involved in the suppression of a cilia assembly program. CEP97 is required for correct spindle formation and has a role in cytokinesis. It is required for recruitment of CEP110 to the centrosome. This protein has a calculated MW ~97 kDa, although the protein migrates at a molecular weight of approximate 140 kDa. The protein is highly acidic, with a pI ~4.7, providing a potential explanation for its reduced mobility in SDS-PAGE. [PMID: 17719545]. This is a rabbit polyclonal antibody raised against the C terminus of human CEP97.

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