验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HL-60 cells, PC-3 cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human liver tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22050-1-AP targets CEP97 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, canine samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, canine |
| 文献引用反应性 | human, mouse, drosophila |
| 免疫原 |
CatNo: Ag16995 Product name: Recombinant human CEP97 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 506-865 aa of BC041085 Sequence: ESTEQKQSDIKKPENTQPENKETISQATSEKLPMILTQRSVALGQDKVALQKLNDAATKLQACWRGFYARNYNPQAKDVRYEIRLRRMQEHIVCLTDEIRRLRKERDEERIKKFVQEEAFRFLWNQVRSLQVWQQTVDQRLSSWHTDVPPISSTLVPSKHPLFTQSQESSCDQNADWFIASDVAPQEKSLPEFPDSGFHSSLTEQVHSLQHSLDFEKSSTEGSESSIMGNSIDTVRYGKESDLGDVSEEHGEWNKESSNNEQDNSLLEQYLTSVQQLEDADERTNFDTETRDSKLHIACFPVQLDTLSDGASVDESHGISPPLQGEISQTQENSKLNAEVQGQQPECDSTFQLLHVGVTV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | centrosomal protein 97kDa |
| 别名 | centrosomal protein 97kDa, Centrosomal protein of 97 kDa, Leucine-rich repeat and IQ domain-containing protein 2, LRRIQ2 |
| 计算分子量 | 865 aa, 97 kDa |
| 观测分子量 | 130-140 kDa |
| GenBank蛋白编号 | BC041085 |
| 基因名称 | CEP97 |
| Gene ID (NCBI) | 79598 |
| RRID | AB_11182378 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IW35 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEP97, also named as LRRIQ2, contains one IQ domain, eight LRR (leucine-rich) repeats and one LRRCT domain. It collaborates with CEP110, being involved in the suppression of a cilia assembly program. CEP97 is required for correct spindle formation and has a role in cytokinesis. It is required for recruitment of CEP110 to the centrosome. This protein has a calculated MW ~97 kDa, although the protein migrates at a molecular weight of approximate 140 kDa. The protein is highly acidic, with a pI ~4.7, providing a potential explanation for its reduced mobility in SDS-PAGE. [PMID: 17719545]. This is a rabbit polyclonal antibody raised against the C terminus of human CEP97.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CEP97 antibody 22050-1-AP | Download protocol |
| IHC protocol for CEP97 antibody 22050-1-AP | Download protocol |
| IP protocol for CEP97 antibody 22050-1-AP | Download protocol |
| WB protocol for CEP97 antibody 22050-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Time-series reconstruction of the molecular architecture of human centriole assembly | ||
Cell Res NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis. | ||
Protein Cell Neddylation inhibitor MLN4924 suppresses cilia formation by modulating AKT1. | ||
Nat Commun M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole. | ||
J Clin Invest Primary cilia formation requires the Leigh syndrome-associated mitochondrial protein NDUFAF2 | ||
Cell Death Dis TUFT1 regulates cancer progression by suppressing centrosome amplification and mitotic spindle multipolarity |
