验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68112 targets CEP89, CCDC123 in IF/ICC applications and shows reactivity with Human, mouse samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, mouse |
| 免疫原 | CEP89, CCDC123 fusion protein Ag28339 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | coiled-coil domain containing 123 |
| 别名 | CCDC123, FLJ14640 |
| 计算分子量 | 783 aa, 90 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC136328 |
| 基因名称 | CEP89 |
| Gene ID (NCBI) | 84902 |
| RRID | AB_3084402 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q96ST8 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCDC123 (as known as CEP123), also named as CEP89, encodes for a protein required for ciliogenesis. It plays a role in mitochondrial metabolism by modulating complex IV activity. It has been shown that CEP123 is localized to the distal appendages of the mother centriolecep and the localization of CEP123 is cell cycle-dependent with its levels decreasing during mitosis. CEP123 depletion can cause defects in ciliary vesicle formationcep and prevent the formation of a ciliary vesicle at the distal end of the mother centriole. It is possible that CEP123 is involved in regulating the recruitment of membranes to the centrosome through its interaction with CEP290 (PMID:23575228, 23789104, 23348840).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CEP89, CCDC123 antibody CL488-68112 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |