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验证数据展示

  • WB analysis using 20022-1-AP (same clone as 20022-1-PBS)

    WB analysis using 20022-1-AP (same clone as 20022-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20022-1-AP (CEP350-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20022-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 20022-1-AP (same clone as 20022-1-PBS)

    IHC staining of mouse liver using 20022-1-AP (same clone as 20022-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20022-1-AP (CEP350-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20022-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 20022-1-AP (same clone as 20022-1-PBS)

    IF Staining of A431 using 20022-1-AP (same clone as 20022-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CEP350-Specific antibody (20022-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20022-1-PBS in a different storage buffer formulation.

CEP350-Specific Polyclonal antibody, PBS Only

CEP350-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20022-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Cep350, GM133, Centrosome-associated protein of 350 kDa, Centrosome-associated protein 350, centrosomal protein 350kDa

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20022-1-PBS targets CEP350-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 centrosomal protein 350kDa
别名 Cep350, GM133, Centrosome-associated protein of 350 kDa, Centrosome-associated protein 350, centrosomal protein 350kDa
计算分子量 351 kDa
观测分子量245 kDa
GenBank蛋白编号NM_014810
基因名称 CEP350
Gene ID (NCBI) 9857
RRIDAB_11232427
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5VT06
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CEP350, or Centrosomal Protein 350 kDa, also named CAP350, FLJ38282, FLJ44058, GM133, and KIAA0480, is a protein that plays a crucial role in the formation and maintenance of the centrosome, which is essential for cell division. The molecular weight of CEP350 varies depending on the isoforms and post-translational modifications. the molecular weight of CEP350 can range from 200-245 kDa, 143 kDa, to 65 kDa. These variations in molecular weight are likely due to different isoforms of the protein that arise from alternative splicing or other post-translational modifications.

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