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验证数据展示

  • WB analysis of HeLa using 22490-1-AP (same clone as 22490-1-PBS)

    WB analysis of HeLa using 22490-1-AP (same clone as 22490-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 22490-1-AP (CEP290 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22490-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 22490-1-AP (same clone as 22490-1-PBS)

    IF Staining of HeLa using 22490-1-AP (same clone as 22490-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22490-1-AP (CEP290 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22490-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 22490-1-AP (same clone as 22490-1-PBS)

    IF Staining of MCF-7 using 22490-1-AP (same clone as 22490-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CEP290 antibody (22490-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 22490-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 22490-1-AP (same clone as 22490-1-PBS)

    IP experiment of HeLa using 22490-1-AP (same clone as 22490-1-PBS)

    IP result of anti-CEP290 (IP:22490-1-AP, 4ug; Detection:22490-1-AP 1:3000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 22490-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 22490-1-AP (same clone as 22490-1-PBS)

    IHC staining of human prostate cancer using 22490-1-AP (same clone as 22490-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22490-1-AP (CEP290 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22490-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CEP290 Polyclonal antibody, PBS Only

CEP290 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 22490-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

CT87, Centrosomal protein of 290 kDa, Cancer/testis antigen 87, BBS14, Bardet-Biedl syndrome 14 protein

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

22490-1-PBS targets CEP290 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag17457

Product name: Recombinant human CEP290 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-237 aa of BC008641

Sequence: QMDSDEMKKILAENSRKITVLQVNEKSLIRQYTTLVELERQLRKENEKQKNELLSMEAEVCEKIGCLQRFKEMAIFKIAALQKVVDNSVSLSELELANKQYNELTAKYRDILQKDNMLVQRTSNLEHLECENISLKEQVESINKELEITKEKLHTIEQAWEQETKLGNESSMDKAKKSITNSDIVSISKKITMLEMKELNERQRAEHCQKMYEHLRTSLKQMEERNFELETKFAEV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 centrosomal protein 290kDa
别名 CT87, Centrosomal protein of 290 kDa, Cancer/testis antigen 87, BBS14, Bardet-Biedl syndrome 14 protein
计算分子量 2479 aa, 290 kDa
观测分子量 290 kDa
GenBank蛋白编号BC008641
基因名称 CEP290
Gene ID (NCBI) 80184
RRIDAB_10973679
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15078
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Studies in the Chlamydomonas model system has shown that the ciliary protein CEP290 is a critical component of these Y-link junctions. Additionally, numerous studies have demonstrated that CEP290's function is critical for IFT-in CEP290 knockdown experiments, many proteins that would normally localize to the cilium fail to do so, and cilium formation is disrupted or absent. Mutations in CEP290 are accountable for cases of nephronophthisis, Leber congenital amaurosis and Joubert syndrome. In IF analtsis of HeLa cells, the white arrows show centrosome and cilium staining. Two isoforms were produced by alternative splicing with predicted MW of 290 and 180 kDa according to UniProt. Catalog#22490-1-AP can recognise both.

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