验证数据展示

IF Staining of HeLa using 22227-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP164 antibody (22227-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP experiment of RPE1 cells using 22227-1-AP
WB results of CEP164 (22227-1-AP, 1:500) with RPE1 cells by Laboratory of Protein Dynamics and Signaling; Center for Cancer Research, National Cancer Institute.
IF Staining of HeLa cells using 22227-1-AP
IF results of CEP164 (22227-1-AP, 1:1000) with HeLa cells (1.5% formaldehyde, 10 min RT) by Laboratory of Protein Dynamics and Signaling; Center for Cancer Research, National Cancer Institute.
IF Staining of HeLa using 22227-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP164 antibody (22227-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
WB analysis of HEK-293 using 22227-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22227-1-AP (CEP164 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 22227-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22227-1-AP (CEP164 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of hTERT-RPE1 using 22227-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed hTERT-RPE1 cells using CEP164 antibody (22227-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC staining of human colon using 22227-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 22227-1-AP (CEP164 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO验证

CEP164 Polyclonal antibody

CEP164 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 22227-1-AP

产品说明书

引用文献(74)

宿主/亚型

Rabbit / IgG

种属反应性

human, canine and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

3F2B7, CEP 164, KIAA1052, centrosomal protein 164kDa, NPHP15

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (74)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HEK-293 cells
Positive IP detected in	RPE1 cells
Positive IHC detected in	human colon tissue, human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, A549 cells, PC-3 cells, MDCK cells, hTERT-RPE1 cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 11 publications below
IHC	See 1 publications below
IF	See 58 publications below
IP	See 1 publications below

产品信息

22227-1-AP targets CEP164 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, canine samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP
经测试反应性	human, canine
文献引用反应性	human, mouse
免疫原	CatNo: Ag17570 Product name: Recombinant human CEP164 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-112 aa of BC000602 Sequence: MAGRPLRIGDQLVLEEDYDETYIPSEQEILEFAREIGIDPIKEPELMWLAREGIVAPLPGEWKPCQDITGDIYYFNFANGQSMWDHPCDEHYRSLVIQERAKLSTSGAIKKK 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	centrosomal protein 164kDa
别名	3F2B7, CEP 164, KIAA1052, centrosomal protein 164kDa, NPHP15
计算分子量	1460 aa, 164 kDa
观测分子量	164 kDa
GenBank蛋白编号	BC000602
基因名称	CEP164
Gene ID (NCBI)	22897
RRID	AB_2651175
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q9UPV0
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

CEP164, also called KIAA1052 or NPHP15, is a 1460 amino acid protein containing 1 WW domain. CEP164 localizes in the microtubule organizing center and is expressed in several cell lines. CEP164 plays a role in microtubule organization and/or maintenance for the formation of primary cilia, a microtubule-based structure that protrudes from the surface of epithelial cells. CEP164 plays a critical role in the G2/M checkpoint and nuclear divisions. The expression of CEP164 is normally limited to the mother centriole, and CEP164 can be used as a useful marker for mother centriole.

实验方案

Product Specific Protocols
IF protocol for CEP164 antibody 22227-1-AP	Download protocol
IHC protocol for CEP164 antibody 22227-1-AP	Download protocol
WB protocol for CEP164 antibody 22227-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	IF	Cell Res NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis. Authors - Min Liu View Article
human	IF	Nat Commun M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole. Authors - Ning Huang View Article
		Nat Commun Sub-centrosomal mapping identifies augmin-γTuRC as part of a centriole-stabilizing scaffold. Authors - Nina Schweizer View Article
		Nat Commun Genetic link between renal birth defects and congenital heart disease. Authors - Jovenal T San Agustin View Article
human	WB	Nat Commun DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6. Authors - Xiaowei Xu View Article
human	IF	EMBO J ANKRD26 recruits PIDD1 to centriolar distal appendages to activate the PIDDosome following centrosome amplification. Authors - Lauren T Evans View Article

验证数据展示

IF Staining of HeLa using 22227-1-AP

IP experiment of RPE1 cells using 22227-1-AP

IF Staining of HeLa cells using 22227-1-AP

IF Staining of HeLa using 22227-1-AP

WB analysis of HEK-293 using 22227-1-AP

IHC staining of human colon using 22227-1-AP

IF Staining of hTERT-RPE1 using 22227-1-AP

IHC staining of human colon using 22227-1-AP

CEP164 Polyclonal antibody

CEP164 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.

M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole.

Sub-centrosomal mapping identifies augmin-γTuRC as part of a centriole-stabilizing scaffold.

Genetic link between renal birth defects and congenital heart disease.

DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6.

ANKRD26 recruits PIDD1 to centriolar distal appendages to activate the PIDDosome following centrosome amplification.