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验证数据展示

  • IHC staining of human liver using 30210-1-AP (same clone as 30210-1-PBS)

    IHC staining of human liver using 30210-1-AP (same clone as 30210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30210-1-AP (CEP162 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30210-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CEP162 antibody included
  • IHC staining of human liver using 30210-1-AP (same clone as 30210-1-PBS)

    IHC staining of human liver using 30210-1-AP (same clone as 30210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30210-1-AP (CEP162 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30210-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CEP162 antibody included
  • WB analysis using 30210-1-AP (same clone as 30210-1-PBS)

    WB analysis using 30210-1-AP (same clone as 30210-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30210-1-AP (CEP162 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30210-1-PBS in a different storage buffer formulation.

CEP162 Polyclonal antibody, PBS Only

CEP162 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30210-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CEP162 Ready-To-Use IHC Kit. CEP162 primary antibody included.

产品信息

30210-1-PBS targets CEP162 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag32953

Product name: Recombinant human CEP162 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 241-438 aa of NM_001286206.1

Sequence: VLLDSLDSVAEVNLDEQDKITPKPRCLPEMTENEMTGTGVSYGQSSSDVEALHQAYCHIAHSLGDEDKQKIESNTVEDIKSSVKGHPQENEENSKNISTMESDLPTVEELMKPIRIDSFGISGFDLQPVSSEKVAERKETEFFSSLPLKMNPNILSQDSQHVNLFFDKNDENVILQKTTNESMENSCPQVTEVTATEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA1009
别名
计算分子量162 kDa
观测分子量180 kDa
GenBank蛋白编号NM_001286206.1
基因名称 CEP162
Gene ID (NCBI) 22832
RRIDAB_2935531
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5TB80
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CEP162, also known as KIAA1009 and QN1, was found in organisms ranging from trypanosomes to vertebrates. A role for CEP162, involved in required to promote assembly of the transition zone in primary cilia. CEP162 acts by specifically recognizing and binding the axonemal microtubule. CEP162 localizes to the distal ends of centrioles before ciliogenesis and directly binds to axonemal microtubule, thereby promoting and restricting transition zone formation specifically at the cilia base.

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