• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CEP162 Polyclonal antibody

CEP162 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 30210-1-AP

Print datasheet

Synonyms

C6orf84, CEP162, FLJ13551, KIAA1009, Protein QN1 homolog, QN1

验证数据展示

  • IHC staining of human liver using 30210-1-AP

    IHC staining of human liver using 30210-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30210-1-AP (CEP162 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEP162 antibody included
  • IHC staining of human liver using 30210-1-AP

    IHC staining of human liver using 30210-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30210-1-AP (CEP162 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEP162 antibody included
  • WB analysis using 30210-1-AP

    WB analysis using 30210-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30210-1-AP (CEP162 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman liver tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CEP162 Ready-To-Use IHC Kit. CEP162 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30210-1-AP targets CEP162 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human
Immunogen CEP162 fusion protein Ag32953 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA1009
Synonyms C6orf84, CEP162, FLJ13551, KIAA1009, Protein QN1 homolog, QN1
Calculated Molecular Weight162 kDa
Observed Molecular Weight180 kDa
GenBank Accession NumberNM_001286206.1
Gene Symbol KIAA1009
Gene ID (NCBI) 22832
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5TB80
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP162, also known as KIAA1009 and QN1, was found in organisms ranging from trypanosomes to vertebrates. A role for CEP162, involved in required to promote assembly of the transition zone in primary cilia. CEP162 acts by specifically recognizing and binding the axonemal microtubule. CEP162 localizes to the distal ends of centrioles before ciliogenesis and directly binds to axonemal microtubule, thereby promoting and restricting transition zone formation specifically at the cilia base.

实验方案

Product Specific Protocols
WB protocol for CEP162 antibody 30210-1-APDownload protocol
IHC protocol for CEP162 antibody 30210-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols