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验证数据展示

  • IHC staining of human liver using 30210-1-AP

    IHC staining of human liver using 30210-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30210-1-AP (CEP162 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEP162 antibody included
  • IHC staining of human liver using 30210-1-AP

    IHC staining of human liver using 30210-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30210-1-AP (CEP162 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEP162 antibody included
  • WB analysis using 30210-1-AP

    WB analysis using 30210-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30210-1-AP (CEP162 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CEP162 Polyclonal antibody

CEP162 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30210-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

C6orf84, CEP162, FLJ13551, KIAA1009, Protein QN1 homolog, QN1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman liver tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CEP162 Ready-To-Use IHC Kit. CEP162 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30210-1-AP targets CEP162 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 CEP162 fusion protein Ag32953 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA1009
别名 C6orf84, CEP162, FLJ13551, KIAA1009, Protein QN1 homolog, QN1
计算分子量162 kDa
观测分子量180 kDa
GenBank蛋白编号NM_001286206.1
基因名称 CEP162
Gene ID (NCBI) 22832
RRIDAB_2935531
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5TB80
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP162, also known as KIAA1009 and QN1, was found in organisms ranging from trypanosomes to vertebrates. A role for CEP162, involved in required to promote assembly of the transition zone in primary cilia. CEP162 acts by specifically recognizing and binding the axonemal microtubule. CEP162 localizes to the distal ends of centrioles before ciliogenesis and directly binds to axonemal microtubule, thereby promoting and restricting transition zone formation specifically at the cilia base.

实验方案

Product Specific Protocols
WB protocol for CEP162 antibody 30210-1-APDownload protocol
IHC protocol for CEP162 antibody 30210-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols