验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells, Jurkat cells, MOLT-4 cells, K-562 cells, IMR-32 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60468-1-Ig targets CEP120 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | CEP120 fusion protein Ag33947 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | centrosomal protein 120kDa |
| 别名 | FLJ36090, Coiled-coil domain-containing protein 100, Centrosomal protein of 120 kDa, centrosomal protein 120kDa, CCDC100 |
| 计算分子量 | 986 aa, 113 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC040527 |
| 基因名称 | CEP120 |
| Gene ID (NCBI) | 153241 |
| RRID | AB_3670261 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8N960 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEP120 encodes a protein that functions in the microtubule-dependent coupling of the nucleus and the centrosome. It is involved in regulating centrosome-mediated interkinetic nuclear migration of neural progenitors (PMID: 27185865, 23857771). CEP120, SPICE1, and CPAP are required for centriole elongation and for the recruitment of distal microtubule-capping proteins and CEP135 to procentrioles (PMID: 23810536).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CEP120 antibody 60468-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |