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CEP120 Polyclonal antibody

CEP120 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24067-1-AP

Print datasheet

Synonyms

CCDC100, centrosomal protein 120kDa, Centrosomal protein of 120 kDa, CEP120

验证数据展示

  • WB analysis of mouse lung using 24067-1-AP

    WB analysis of mouse lung using 24067-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 24067-1-AP (CEP120 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 24067-1-AP

    WB analysis using 24067-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24067-1-AP (CEP120 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 24067-1-AP

    IF Staining of HeLa using 24067-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP120 antibody (24067-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

  • IHC staining of human pancreas using 24067-1-AP

    IHC staining of human pancreas using 24067-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24067-1-AP (CEP120 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas using 24067-1-AP

    IHC staining of human pancreas using 24067-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24067-1-AP (CEP120 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 24067-1-AP

    IF Staining of HeLa using 24067-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP120 antibody (24067-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, mouse lung tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24067-1-AP targets CEP120 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen CEP120 fusion protein Ag21165 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name centrosomal protein 120kDa
Synonyms CCDC100, centrosomal protein 120kDa, Centrosomal protein of 120 kDa, CEP120
Calculated Molecular Weight 986 aa, 113 kDa
Observed Molecular Weight120-130 kDa
GenBank Accession NumberBC040527
Gene Symbol CEP120
Gene ID (NCBI) 153241
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N960
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP120 encodes a protein that functions in the microtubule-dependent coupling of the nucleus and the centrosome. It is involved in regulating centrosome-mediated interkinetic nuclear migration of neural progenitors (PubMed: 27185865, PubMed: 23857771). CEP120, SPICE1 and CPAP are required for centriole elongation and for recruitment of distal microtubule-capping proteins and CEP135 to procentrioles (PubMed: 23810536). Cep120 and Taccs are required for the neural progenitor pool during mouse neocortical development (PubMed: 17920017).

实验方案

Product Specific Protocols
WB protocol for CEP120 antibody 24067-1-APDownload protocol
IHC protocol for CEP120 antibody 24067-1-APDownload protocol
IF protocol for CEP120 antibody 24067-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols