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验证数据展示

  • WB analysis using 19840-1-AP (same clone as 19840-1-PBS)

    WB analysis using 19840-1-AP (same clone as 19840-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19840-1-AP (CENPM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19840-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 19840-1-AP (same clone as 19840-1-PBS)

    IF Staining of NIH/3T3 using 19840-1-AP (same clone as 19840-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CENPM antibody (19840-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 19840-1-PBS in a different storage buffer formulation.

CENPM Polyclonal antibody, PBS Only

CENPM Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 19840-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

19840-1-PBS targets CENPM in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13843

Product name: Recombinant human CENPM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-180 aa of BC000705

Sequence: MSVLRPLDKLPGLNTATILLVGTEDALLQQLADSMLKEDCASELKVHLAKSLPLPSSVNRPRIDLIVFVVNLHSKYSLQNTEESLRHVDASFFLGKVCFLATGAGRESHCSIHRHTVVKLAHTYQSPLLYCDLEVEGFRATMAQRLVRVLQICAGHVPGVSALNLLSLLRSSEGPSLEDL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 centromere protein M
别名
计算分子量 180 aa, 20 kDa
观测分子量20 kDa
GenBank蛋白编号BC000705
基因名称 CENPM
Gene ID (NCBI) 79019
RRIDAB_3661902
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NSP4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Centromere protein M (CENPM), also referred as proliferation associated nuclear element 1 (PANE1), is one of the components of a complex that plays a central role in kinetochore protein assembly, mitotic progression and chromosome segregation. The expression of CENPM and centromere assembly should be controlled closely during the cell cycle, and mistakes in this regulation may result in aneuploidy (PMID: 32705259). CENPM has been reported as a novel biomarker of hepatocellular carcinoma, melanoma and bladder cancer (PMID: 31703591).

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