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验证数据展示

  • WB analysis using 19840-1-AP

    WB analysis using 19840-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19840-1-AP (CENPM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 19840-1-AP

    IF Staining of NIH/3T3 using 19840-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CENPM antibody (19840-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

CENPM Polyclonal antibody

CENPM Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 19840-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

ICEN39, Centromere protein M, CENP-M, CENP M, C22orf18

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, HeLa cells, Raji cells
Positive IF/ICC detected inNIH/3T3 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19840-1-AP targets CENPM in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CENPM fusion protein Ag13843 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 centromere protein M
别名 ICEN39, Centromere protein M, CENP-M, CENP M, C22orf18
计算分子量 180 aa, 20 kDa
观测分子量20 kDa
GenBank蛋白编号BC000705
基因名称 CENPM
Gene ID (NCBI) 79019
RRIDAB_3661902
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NSP4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Centromere protein M (CENPM), also referred as proliferation associated nuclear element 1 (PANE1), is one of the components of a complex that plays a central role in kinetochore protein assembly, mitotic progression and chromosome segregation. The expression of CENPM and centromere assembly should be controlled closely during the cell cycle, and mistakes in this regulation may result in aneuploidy (PMID: 32705259). CENPM has been reported as a novel biomarker of hepatocellular carcinoma, melanoma and bladder cancer (PMID: 31703591).

实验方案

Product Specific Protocols
WB protocol for CENPM antibody 19840-1-APDownload protocol
IF protocol for CENPM antibody 19840-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols