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Host
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验证数据展示

  • FC experiment of mouse splenocytes using CL647-98185

    FC experiment of mouse splenocytes using CL647-98185

    1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD45R (B220) (RA3-6B2) (CL488-65139, Clone: RA3-6B2) and 0.25 ug CoraLite® Plus 647 Anti-Mouse CEACAM1/CD66a Rabbit RecAb (CL647-98185, Clone: 241856H5) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of mouse splenocytes using CL647-98185

    FC experiment of mouse splenocytes using CL647-98185

    1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CEACAM1/CD66a Rabbit RecAb (CL647-98185, Clone: 241856H5) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CoraLite® Plus 647 Anti-Mouse CEACAM1/CD66a Rabbit Recombinant Antibody

CEACAM1/CD66a Uni-rAbTM Recombinant Antibody for FC
Cat No. CL647-98185

产品说明书

CloneNo. 241856H5

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

241856H5, Bgp, Bgp1, BGP-1, Biliary glycoprotein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 98185-1-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-98185 targets CEACAM1/CD66a in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原 CEACAM1/CD66a fusion protein Eg1423 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 carcinoembryonic antigen-related cell adhesion molecule 1
别名 241856H5, Bgp, Bgp1, BGP-1, Biliary glycoprotein 1
计算分子量57 kDa
基因名称 Ceacam1
Gene ID (NCBI) 26365
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP31809-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CEACAM1/CD66a antibody CL647-98185Download protocol
Standard Protocols
Click here to view our Standard Protocols