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验证数据展示

  • WB analysis of MCF-7 using 83653-5-RR

    WB analysis of MCF-7 using 83653-5-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83653-5-RR (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83653-5-RR

    IHC staining of human ovary cancer using 83653-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83653-5-RR (CEA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • IHC staining of human ovary cancer using 83653-5-RR

    IHC staining of human ovary cancer using 83653-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83653-5-RR (CEA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • IF Staining of HT-29 using 83653-5-RR

    IF Staining of HT-29 using 83653-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CEA antibody (83653-5-RR, Clone: 240666B12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83653-5-RR

    Affinity and Kinetic Characterization of 83653-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83653-5-RR against Human CEA were performed. The affinity constant is 0.734 nM.

CEA/CD66e Recombinant monoclonal antibody

CEA/CD66e Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83653-5-RR

产品说明书

CloneNo. 240666B12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CEA, CEACAM5, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, human saliva
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy CEA/CD66e Ready-To-Use IHC Kit. CEA/CD66e primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83653-5-RR targets CEA/CD66e in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

Fusion Protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 carcinoembryonic antigen-related cell adhesion molecule 5
别名 CEA, CEACAM5, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen
计算分子量 77 kDa
观测分子量200 kDa
GenBank蛋白编号NM_004363
基因名称 CEA
Gene ID (NCBI) 1048
RRIDAB_3671261
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP06731-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis. This antibody is raised against 35-685 aa of human CEA/CD66e and can cross-react with CEACAM1/CD66a and CEACAM3/CD66d.

实验方案

Product Specific Protocols
IF protocol for CEA/CD66e antibody 83653-5-RRDownload protocol
IHC protocol for CEA/CD66e antibody 83653-5-RRDownload protocol
WB protocol for CEA/CD66e antibody 83653-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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