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验证数据展示

  • IHC staining of human colon cancer using 68377-1-Ig

    IHC staining of human colon cancer using 68377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEACAM5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • IHC staining of human colon cancer using 68377-1-Ig

    IHC staining of human colon cancer using 68377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEACAM5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • WB analysis using 68377-1-Ig

    WB analysis using 68377-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68377-1-Ig (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68377-1-Ig

    WB analysis using 68377-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68377-1-Ig (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 68377-1-Ig

    IHC staining of human stomach cancer using 68377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68377-1-Ig (CEA/CD66e antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human colorectal cancer using 68377-1-Ig

    IHC staining of human colorectal cancer using 68377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 68377-1-Ig (CEA/CD66e antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human cervical cancer using 68377-1-Ig

    IHC staining of human cervical cancer using 68377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68377-1-Ig (CEA/CD66e antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human pancreas cancer using 68377-1-Ig

    IHC staining of human pancreas cancer using 68377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68377-1-Ig (CEA/CD66e antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human large intestine using 68377-1-Ig

    IHC staining of human large intestine using 68377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human large intestine tissue slide using 68377-1-Ig (CEA/CD66e antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human appendicitis using 68377-1-Ig

    IHC staining of human appendicitis using 68377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68377-1-Ig (CEA/CD66e antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human appendicitis using 68377-1-Ig

    IHC staining of human appendicitis using 68377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68377-1-Ig (CEA/CD66e antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • IHC staining of human cervical cancer using 68377-1-Ig

    IHC staining of human cervical cancer using 68377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68377-1-Ig (CEA/CD66e antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • IHC staining of human cervical cancer using 68377-1-Ig

    IHC staining of human cervical cancer using 68377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68377-1-Ig (CEA/CD66e antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • IHC staining of human pancreas using 68377-1-Ig

    IHC staining of human pancreas using 68377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 68377-1-Ig (CEA/CD66e antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • IHC staining of human large intestine using 68377-1-Ig

    IHC staining of human large intestine using 68377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human large intestine tissue slide using 68377-1-Ig (CEA/CD66e antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • IHC staining of human colorectal cancer using 68377-1-Ig

    IHC staining of human colorectal cancer using 68377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 68377-1-Ig (CEA/CD66e antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • IHC staining of human pancreas cancer using 68377-1-Ig

    IHC staining of human pancreas cancer using 68377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68377-1-Ig (CEA/CD66e antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • IHC staining of human stomach cancer using 68377-1-Ig

    IHC staining of human stomach cancer using 68377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68377-1-Ig (CEA/CD66e antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included
  • IHC staining of human stomach cancer using 68377-1-Ig

    IHC staining of human stomach cancer using 68377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68377-1-Ig (CEA/CD66e antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEA/CD66e antibody included

CEA/CD66e Monoclonal antibody

CEA/CD66e Monoclonal Antibody for WB, IHC, IHC-Autostainer, ELISA
Cat No. 68377-1-Ig

产品说明书

CloneNo. 2B8H6
引用文献(3)

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IHC-Autostainer, ELISA

别名

CEA, 2B8H6, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, CD66e

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHaCaT cells, human saliva, HT-29 cells, human rectum tissue
Positive IHC-Autostainer detected inhuman pancreas cancer tissue, human cervical cancer tissue, human colorectal cancer tissue, human large intestine tissue, human stomach cancer tissue, human appendicitis tissue
Positive IHC detected inhuman colon cancer tissue, human pancreas cancer tissue, human appendicitis tissue, human stomach cancer tissue, human cervical cancer tissue, human colorectal cancer tissue, human pancreas tissue, human large intestine tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CEA/CD66e Ready-To-Use IHC Kit. CEA/CD66e primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)-AUTOSTAINERIHC-AUTOSTAINER : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68377-1-Ig targets CEA/CD66e in WB, IHC, IHC-Autostainer, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IHC-Autostainer, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Eg0247

Product name: Recombinant Human CEA/CD66e protein (hFc Tag)

Source: mammalian cells-derived, pINFUSE-IGSF8-CFc2(A2)

Tag: C-hIGg2fc

Domain: 35-685 aa of BC034671

Sequence: KLTIESTPFNVAEGKEVLLLVHNLPQHLFGYSWYKGERVDGNRQIIGYVIGTQQATPGPAYSGREIIYPNASLLIQNIIQNDTGFYTLHVIKSDLVNEEATGQFRVYPELPKPSISSNNSKPVEDKDAVAFTCEPETQDATYLWWVNNQSLPVSPRLQLSNGNRTLTLFNVTRNDSASYKCETQNPVSARRSDSVILNVLYGPDAPTISPLNTSYRSGENLNLSCHAASNPPAQYSWFVNGTFQQSTQELFIPNITVNNSGSYTCQAHNSDTGLNRTTVTTITVYAEPPKPFITSNNSNPVEDEDAVALTCEPEIQNTTYLWWVNNQSLPVSPRLQLSNDNRTLTLLSVTRNDVGPYECGIQNELSVDHSDPVILNVLYGPDDPTISPSYTYYRPGVNLSLSCHAASNPPAQYSWLIDGNIQQHTQELFISNITEKNSGLYTCQANNSASGHSRTTVKTITVSAELPKPSISSNNSKPVEDKDAVAFTCEPEAQNTTYLWWVNGQSLPVSPRLQLSNGNRTLTLFNVTRNDARAYVCGIQNSVSANRSDPVTLDVLYGPDTPIISPPDSSYLSGANLNLSCHSASNPSPQYSWRINGIPQQHTQVLFIAKITPNNNGTYACFVSNLATGRNNSIVKSITVSASGTSPGLSA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 carcinoembryonic antigen-related cell adhesion molecule 5
别名 CEA, 2B8H6, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, CD66e
计算分子量 77 kDa
观测分子量180-200 kDa
GenBank蛋白编号BC034671
基因名称 CEA
Gene ID (NCBI) 1048
RRIDAB_3085095
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP06731
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.

实验方案

Product Specific Protocols
IHC protocol for CEA/CD66e antibody 68377-1-IgDownload protocol
WB protocol for CEA/CD66e antibody 68377-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Biomark Res

Histone modification-regulated LncRNA DLEU1 interacts with ASCC2/ALKBH3 complex to drive DNA repair, antioxidant homeostasis and glucose metabolism in gastric cancer.

Authors - Xiaoyan Zhang
View Article
humanWB

Cancer Lett

A High-Affinity Antibody-Drug Conjugates Actuximab-MMAE for Potent and Selective Targeting of CEACAM5-Positive Tumors

Authors - Yuqi Hu
View Article
humanIHC

BMC Cancer

Individualized drug screening in cholangiocarcinoma using organoid models and patient-derived tumor xenograft.

Authors - Pinsheng Han
View Article
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