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验证数据展示

  • IHC staining of human lung cancer using 83662-7-RR (same clone as 83662-7-PBS)

    IHC staining of human lung cancer using 83662-7-RR (same clone as 83662-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83662-7-RR (CDK9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83662-7-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK9 antibody included
  • IHC staining of human lung cancer using 83662-7-RR (same clone as 83662-7-PBS)

    IHC staining of human lung cancer using 83662-7-RR (same clone as 83662-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83662-7-RR (CDK9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83662-7-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK9 antibody included
  • IF Staining of HeLa using 83662-7-RR (same clone as 83662-7-PBS)

    IF Staining of HeLa using 83662-7-RR (same clone as 83662-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (83662-7-RR, Clone: 240600D7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83662-7-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83662-7-RR (same clone as 83662-7-PBS)

    IF Staining of HeLa using 83662-7-RR (same clone as 83662-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (83662-7-RR, Clone: 240600D7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83662-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83662-7-RR

    Affinity and Kinetic Characterization of 83662-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83662-7-RR against Human CDK9 were performed. The affinity constant is below 1 pM.

CDK9 Recombinant monoclonal antibody, PBS Only

CDK9 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 83662-7-PBS

产品说明书

CloneNo. 240600D7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

CTK1, Cell division cycle 2-like protein kinase 4, CDC2L4, C-2K, C 2k

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CDK9 Ready-To-Use IHC Kit. CDK9 primary antibody included.

产品信息

83662-7-PBS targets CDK9 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2318

Product name: Recombinant human CDK9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-372 aa of BC001968

Sequence: MAKQYDSVECPFCDEVSKYEKLAKIGQGTFGEVFKARHRKTGQKVALKKVLMENEKEGFPITALREIKILQLLKHENVVNLIEICRTKASPYNRCKGSIYLVFDFCEHDLAGLLSNVLVKFTLSEIKRVMQMLLNGLYYIHRNKILHRDMKAANVLITRDGVLKLADFGLARAFSLAKNSQPNRYTNRVVTLWYRPPELLLGERDYGPPIDLWGAGCIMAEMWTRSPIMQGNTEQHQLALISQLCGSITPEVWPNVDNYELYEKLELVKGQKRKVKDRLKAYVRDPYALDLIDKLLVLDPAQRIDSDDALNHDFFWSDPMPSDLKGMLSTHLTSMFEYLAPPRRKGSQITQQSTNQSRNPATTNQTEFERVF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin-dependent kinase 9
别名 CTK1, Cell division cycle 2-like protein kinase 4, CDC2L4, C-2K, C 2k
计算分子量 372 aa, 43 kDa
观测分子量 38-42 kDa, 55 kDa
GenBank蛋白编号BC001968
基因名称 CDK9
Gene ID (NCBI) 1025
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP50750
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243).

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