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验证数据展示

  • IHC staining of human lung cancer using 83662-7-RR

    IHC staining of human lung cancer using 83662-7-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83662-7-RR (CDK9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK9 antibody included
  • IHC staining of human lung cancer using 83662-7-RR

    IHC staining of human lung cancer using 83662-7-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83662-7-RR (CDK9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK9 antibody included
  • IF Staining of HeLa using 83662-7-RR

    IF Staining of HeLa using 83662-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (83662-7-RR, Clone: 240600D7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 83662-7-RR

    IF Staining of HeLa using 83662-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (83662-7-RR, Clone: 240600D7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83662-7-RR

    Affinity and Kinetic Characterization of 83662-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83662-7-RR against Human CDK9 were performed. The affinity constant is below 1 pM.

CDK9 Recombinant antibody

CDK9 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 83662-7-RR

产品说明书

CloneNo. 240600D7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

CTK1, Cell division cycle 2-like protein kinase 4, CDC2L4, C-2K, C 2k

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDK9 Ready-To-Use IHC Kit. CDK9 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83662-7-RR targets CDK9 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CDK9 fusion protein Ag2318 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin-dependent kinase 9
别名 CTK1, Cell division cycle 2-like protein kinase 4, CDC2L4, C-2K, C 2k
计算分子量 372 aa, 43 kDa
观测分子量 38-42 kDa, 55 kDa
GenBank蛋白编号BC001968
基因名称 CDK9
Gene ID (NCBI) 1025
RRIDAB_3671269
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP50750
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243).

实验方案

Product Specific Protocols
IHC protocol for CDK9 antibody 83662-7-RRDownload protocol
IF protocol for CDK9 antibody 83662-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols