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验证数据展示

  • WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

    WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

  • WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

    WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66278-1-Ig (same clone as 66278-1-PBS)

    WB analysis of HEK-293 using 66278-1-Ig (same clone as 66278-1-PBS)

    WB result of CDK6 antibody (66278-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK6 transfected HEK-293 cells. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 66278-1-Ig (same clone as 66278-1-PBS)

    IHC staining of human skin cancer using 66278-1-Ig (same clone as 66278-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66278-1-Ig (CDK6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK6 antibody included
  • IF Staining of HeLa using 66278-1-Ig (same clone as 66278-1-PBS)

    IF Staining of HeLa using 66278-1-Ig (same clone as 66278-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66278-1-Ig(CDK6 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

  • WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

    WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CDK6 Monoclonal antibody, PBS Only

CDK6 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66278-1-PBS

产品说明书

CloneNo. 4B9C11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

EC:2.7.11.22, Cyclin-dependent kinase 6, Cyclin dependent kinase 6, Cell division protein kinase 6, CDKN6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CDK6 Ready-To-Use IHC Kit. CDK6 primary antibody included.

产品信息

66278-1-PBS targets CDK6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CDK6 fusion protein Ag5600 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cyclin-dependent kinase 6
别名 EC:2.7.11.22, Cyclin-dependent kinase 6, Cyclin dependent kinase 6, Cell division protein kinase 6, CDKN6
计算分子量 326 aa, 36 kDa
观测分子量 36-40 kDa
GenBank蛋白编号BC027989
基因名称 CDK6
Gene ID (NCBI) 1021
RRIDAB_2881661
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ00534
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739).