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验证数据展示

  • WB analysis using 86177-2-RR (same clone as 86177-2-PBS)

    WB analysis using 86177-2-RR (same clone as 86177-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86177-2-RR (CDK5RAP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86177-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 86177-2-RR (same clone as 86177-2-PBS)

    IHC staining of human colon cancer using 86177-2-RR (same clone as 86177-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86177-2-RR (CDK5RAP3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86177-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK5RAP3 antibody included
  • IF Staining of HepG2 using 86177-2-RR (same clone as 86177-2-PBS)

    IF Staining of HepG2 using 86177-2-RR (same clone as 86177-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK5RAP3 antibody (86177-2-RR, Clone: 250801G11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86177-2-PBS in a different storage buffer formulation.

CDK5RAP3 Recombinant monoclonal antibody, PBS Only

CDK5RAP3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86177-2-PBS

产品说明书

CloneNo. 250801G11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

C53, CDK5 activator-binding protein C53, CDK5 regulatory subunit-associated protein 3, HSF 27, HSF27

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CDK5RAP3 Ready-To-Use IHC Kit. CDK5RAP3 primary antibody included.

产品信息

86177-2-PBS targets CDK5RAP3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1474

Product name: Recombinant human CDK5RAP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC009957

Sequence: MEDHQHVPIDIQTSKLLDWLVDRRHCSLKWQSLVLTIREKINAAIQDMPESEEIAQLLSGSYIHYFHCLRILDLLKGTEASTKNIFGRYSSQRMKDWQEIIALYEKDNTYLVELSSLLVRNVNYEIPSLKKQIAKCQQLQQEYSRKEEECQAGAAEMREQFYHSCKQYGITGENVRGELLALVKDLPSQLAEIGAAAQQSLGEAIDVYQASVGFVCESPTEQVLPMLRFVQKRGNSTVYEWRTGTEPSVVERPHLEELPEQVAEDAIDWGDFGVEAVSEGTDSGISAEAAGIDWGIFPES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CDK5 regulatory subunit associated protein 3
别名 C53, CDK5 activator-binding protein C53, CDK5 regulatory subunit-associated protein 3, HSF 27, HSF27
计算分子量 57 kDa
观测分子量 65 kDa
GenBank蛋白编号BC009957
基因名称 CDK5RAP3
Gene ID (NCBI) 80279
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96JB5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclin-dependent kinase 5 (CDK5) regulatory subunit associated protein 3 (CDK5RAP3, also named as C53 or LZAP) was initially identified as a binding protein of CDK5 activator p35. CDK5RAP3 is widely expressed in human tissues and broadly located in subcellular compartments. By interacting with other proteins, CDK5RAP3 participates in the regulation of multiple cellular processes including cell cycle, apoptosis, cell invasion, signaling transduction, autophagy, UFMylation and endoplasmic reticulum (ER) stress.

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