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验证数据展示

  • WB analysis using 86177-2-RR

    WB analysis using 86177-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86177-2-RR (CDK5RAP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 86177-2-RR

    IHC staining of human colon cancer using 86177-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86177-2-RR (CDK5RAP3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK5RAP3 antibody included
  • IF Staining of HepG2 using 86177-2-RR

    IF Staining of HepG2 using 86177-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK5RAP3 antibody (86177-2-RR, Clone: 250801G11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CDK5RAP3 Recombinant monoclonal antibody

CDK5RAP3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 86177-2-RR

产品说明书

CloneNo. 250801G11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

C53, CDK5 activator-binding protein C53, CDK5 regulatory subunit-associated protein 3, HSF 27, HSF27

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, A431 cells, HEK-293T cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CDK5RAP3 Ready-To-Use IHC Kit. CDK5RAP3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86177-2-RR targets CDK5RAP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1474

Product name: Recombinant human CDK5RAP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC009957

Sequence: MEDHQHVPIDIQTSKLLDWLVDRRHCSLKWQSLVLTIREKINAAIQDMPESEEIAQLLSGSYIHYFHCLRILDLLKGTEASTKNIFGRYSSQRMKDWQEIIALYEKDNTYLVELSSLLVRNVNYEIPSLKKQIAKCQQLQQEYSRKEEECQAGAAEMREQFYHSCKQYGITGENVRGELLALVKDLPSQLAEIGAAAQQSLGEAIDVYQASVGFVCESPTEQVLPMLRFVQKRGNSTVYEWRTGTEPSVVERPHLEELPEQVAEDAIDWGDFGVEAVSEGTDSGISAEAAGIDWGIFPES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CDK5 regulatory subunit associated protein 3
别名 C53, CDK5 activator-binding protein C53, CDK5 regulatory subunit-associated protein 3, HSF 27, HSF27
计算分子量 57 kDa
观测分子量 65 kDa
GenBank蛋白编号BC009957
基因名称 CDK5RAP3
Gene ID (NCBI) 80279
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96JB5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin-dependent kinase 5 (CDK5) regulatory subunit associated protein 3 (CDK5RAP3, also named as C53 or LZAP) was initially identified as a binding protein of CDK5 activator p35. CDK5RAP3 is widely expressed in human tissues and broadly located in subcellular compartments. By interacting with other proteins, CDK5RAP3 participates in the regulation of multiple cellular processes including cell cycle, apoptosis, cell invasion, signaling transduction, autophagy, UFMylation and endoplasmic reticulum (ER) stress.

实验方案

Product Specific Protocols
IF protocol for CDK5RAP3 antibody 86177-2-RRDownload protocol
IHC protocol for CDK5RAP3 antibody 86177-2-RRDownload protocol
WB protocol for CDK5RAP3 antibody 86177-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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