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验证数据展示

  • WB analysis using 67503-1-Ig (same clone as 67503-1-PBS)

    WB analysis using 67503-1-Ig (same clone as 67503-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67503-1-Ig (CDK5R1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67503-1-Ig (same clone as 67503-1-PBS)

    IHC staining of mouse brain using 67503-1-Ig (same clone as 67503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67503-1-Ig (CDK5R1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK5R1 antibody included
  • IHC staining of mouse brain using 67503-1-Ig (same clone as 67503-1-PBS)

    IHC staining of mouse brain using 67503-1-Ig (same clone as 67503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67503-1-Ig (CDK5R1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK5R1 antibody included
  • WB analysis of mouse cerebellum using 67503-1-Ig (same clone as 67503-1-PBS)

    WB analysis of mouse cerebellum using 67503-1-Ig (same clone as 67503-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67503-1-Ig (CDK5R1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67503-1-PBS in a different storage buffer formulation.

CDK5R1 Monoclonal antibody, PBS Only

CDK5R1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67503-1-PBS

产品说明书

CloneNo. 1A3A5

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat, pig, rabbit

应用

WB, IHC, Indirect ELISA

别名

CDK5 activator 1, CDK5P35, CDK5R, CDK5R1, NCK5A, p23, p25, p35, p35nck5a, TPKII regulatory subunit

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CDK5R1 Ready-To-Use IHC Kit. CDK5R1 primary antibody included.

产品信息

67503-1-PBS targets CDK5R1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag13152

Product name: Recombinant human CDK5R1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 99-307 aa of BC020580

Sequence: AQPPPAQPPAPPASQLSGSQTGGSSSVKKAPHPAVTSAGTPKRVIVQASTSELLRCLGEFLCRRCYRLKHLSPTDPVLWLRSVDRSLLLQGWQDQGFITPANVVFLYMLCRDVISSEVGSDHELQAVLLTCLYLSYSYMGNEISYPLKPFLVESCKEAFWDRCLSVINLMSSKMLQINADPHYFTQVFSDLKNESGQEDKKRLLLGLDR

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cyclin-dependent kinase 5, regulatory subunit 1 (p35)
别名 CDK5 activator 1, CDK5P35, CDK5R, CDK5R1, NCK5A, p23, p25, p35, p35nck5a, TPKII regulatory subunit
计算分子量 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC020580
基因名称 CDK5R1
Gene ID (NCBI) 8851
RRIDAB_2882727
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ15078
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclin dependent kinase-5 (Cdk5), is an atypical but essential member of the Cdk family of proline-directed serine/threonine kinases. CDK5R1 encodes p35, a specific activator of the serine/threonine kinase CDK5, which plays a central role in neuronal migration and differentiation during central nervous system development. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form.

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