验证数据展示
经过测试的应用
| Positive WB detected in | pig cerebellum tissue, pig brain tissue, rat brain tissue, mouse brain tissue, mouse cerebellum tissue |
| Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67503-1-Ig targets CDK5R1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | Human, mouse, rat, pig, rabbit |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag13152 Product name: Recombinant human CDK5R1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 99-307 aa of BC020580 Sequence: AQPPPAQPPAPPASQLSGSQTGGSSSVKKAPHPAVTSAGTPKRVIVQASTSELLRCLGEFLCRRCYRLKHLSPTDPVLWLRSVDRSLLLQGWQDQGFITPANVVFLYMLCRDVISSEVGSDHELQAVLLTCLYLSYSYMGNEISYPLKPFLVESCKEAFWDRCLSVINLMSSKMLQINADPHYFTQVFSDLKNESGQEDKKRLLLGLDR 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cyclin-dependent kinase 5, regulatory subunit 1 (p35) |
| 别名 | CDK5 activator 1, CDK5P35, CDK5R, CDK5R1, NCK5A, p23, p25, p35, p35nck5a, TPKII regulatory subunit |
| 计算分子量 | 34 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC020580 |
| 基因名称 | CDK5R1 |
| Gene ID (NCBI) | 8851 |
| RRID | AB_2882727 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q15078 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cyclin dependent kinase-5 (Cdk5), is an atypical but essential member of the Cdk family of proline-directed serine/threonine kinases. CDK5R1 encodes p35, a specific activator of the serine/threonine kinase CDK5, which plays a central role in neuronal migration and differentiation during central nervous system development. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CDK5R1 antibody 67503-1-Ig | Download protocol |
| WB protocol for CDK5R1 antibody 67503-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun GRAMD1B is a regulator of lipid homeostasis, autophagic flux and phosphorylated tau | ||
J Pharm Anal Histone deacetylase inhibitor pracinostat suppresses colorectal cancer by inducing CDK5-Drp1 signaling-mediated peripheral mitofission | ||
Mol Ther Nucleic Acids Implications of miR-148a-3p/p35/PTEN signaling in tau hyperphosphorylation and autoregulatory feedforward of Akt/CREB in Alzheimer's disease. | ||
Cancer Lett CDK5RAP3 is a novel super-enhancer-driven gene activated by master TFs and regulates ER-Phagy in neuroblastoma | ||
Sci China Life Sci FSD1 inhibits glioblastoma diffuse infiltration through restriction of HDAC6-mediated microtubule deacetylation | ||
Acta Pharm Sin B CDK5-triggered G6PD phosphorylation at threonine 91 facilitating redox homeostasis reveals a vulnerability in breast cancer |
