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验证数据展示

  • WB analysis using 55103-1-AP (same clone as 55103-1-PBS)

    WB analysis using 55103-1-AP (same clone as 55103-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 55103-1-AP (same clone as 55103-1-PBS)

    FC experiment of HeLa using 55103-1-AP (same clone as 55103-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK3 Polyclonal antibody (55103-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 55103-1-AP (same clone as 55103-1-PBS)

    IF Staining of HeLa using 55103-1-AP (same clone as 55103-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK3 antibody (55103-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 55103-1-AP (same clone as 55103-1-PBS)

    IHC staining of human breast cancer using 55103-1-AP (same clone as 55103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55103-1-AP (CDK3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK3 antibody included
  • IHC staining of human breast cancer using 55103-1-AP (same clone as 55103-1-PBS)

    IHC staining of human breast cancer using 55103-1-AP (same clone as 55103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55103-1-AP (CDK3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK3 antibody included

CDK3 Polyclonal antibody, PBS Only

CDK3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 55103-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

CDKN3, EC:2.7.11.22, Cyclin-dependent kinase 3, Cyclin dependent kinase 3, Cell division protein kinase 3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CDK3 Ready-To-Use IHC Kit. CDK3 primary antibody included.

产品信息

55103-1-PBS targets CDK3 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin-dependent kinase 3
别名 CDKN3, EC:2.7.11.22, Cyclin-dependent kinase 3, Cyclin dependent kinase 3, Cell division protein kinase 3
计算分子量 35 kDa
观测分子量 40-45 kDa
GenBank蛋白编号NM_001258
基因名称 CDK3
Gene ID (NCBI) 1018
RRIDAB_10804352
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ00526
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDK3 belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. It is probably involved in the control of the cell cycle. CDK3 interacts with a yet unknown type of cyclin. It can phosphorylate histone H1. Endogenous Cyclin C/Cdk3 complexes are critical to promote exit from quiescence in an Rb-dependent manner. Cyclin C/Cdk3 complexes is a key regulators of cell cycle reentry in human cells. (PMID: 15130482 )The antibody is specific to CDK3.

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