CDK2 Monoclonal antibody

CDK2 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human and More (1)

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

4E2E10

Cat No : 60312-1-Ig

Print datasheet

Synonyms

CDK2, Cell division protein kinase 2, cyclin dependent kinase 2, p33 protein kinase, p33(CDK2)



经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman lymphoma tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

产品信息

60312-1-Ig targets CDK2 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IHC, WB, ELISA
Cited Applications IHC, WB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen CDK2 fusion protein Ag21310 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name cyclin-dependent kinase 2
Synonyms CDK2, Cell division protein kinase 2, cyclin dependent kinase 2, p33 protein kinase, p33(CDK2)
Calculated molecular weight 33 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC003065
Gene symbol CDK2
UniprotP24941
Gene ID (NCBI) 1017
RRIDAB_2881424
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for CDK2 antibody 60312-1-IgDownload protocol
IHC protocol for CDK2 antibody 60312-1-IgDownload protocol
FC protocol for CDK2 antibody 60312-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncotarget

High expression of N-myc (and STAT) interactor predicts poor prognosis and promotes tumor growth in human glioblastoma.

Authors - Delong Meng
humanWB

Oxid Med Cell Longev

MICAL2 Contributes to Gastric Cancer Cell Proliferation by Promoting YAP Dephosphorylation and Nuclear Translocation

Authors - Chenxiang Qi
humanWB

Ecotoxicol Environ Saf

The role of ER stress and ATP/AMPK in oxidative stress meditated hepatotoxicity induced by citrinin.

Authors - Jing Wu
humanWB

J Hepatocell Carcinoma

CYP2C8 Suppress Proliferation, Migration, Invasion and Sorafenib Resistance of Hepatocellular Carcinoma via PI3K/Akt/p27 kip1 Axis

Authors - Xin Zhou
humanWB

Toxicol Lett

The role of the ATM/Chk/P53 pathway in mediating DNA damage in hand-foot syndrome induced by PLD.

Authors - Jie Yang
mouseWB

Immunol Invest

Bovine Milk Exosomes Affect Proliferation and Protect Macrophages against Cisplatin-Induced Cytotoxicity.

Authors - Svjetlana Matic