验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293 cells, HeLa cells, K-562 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 1 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
65182-1-Ig targets CDK1 in WB, FC (Intra) applications and shows reactivity with human, bovine samples.
| 经测试应用 | WB, FC (Intra) Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, bovine |
| 文献引用反应性 | human, sheep |
| 免疫原 |
Bacterially expressed full-length human Cdk1 protein 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cell division cycle 2, G1 to S and G2 to M |
| 别名 | CDC2, CDC28A, CDKN1, Cell division protein kinase 1, Cyclin dependent kinase 1 |
| 计算分子量 | 25 kDa |
| 观测分子量 | 30-34 kDa |
| GenBank蛋白编号 | BC014563 |
| 基因名称 | CDK1 |
| Gene ID (NCBI) | 983 |
| RRID | AB_2918456 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Purified by protein-A affinity chromatography |
| UNIPROT ID | P06493 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDK1 antibody 65182-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Zool Res LncRNA GTL2 regulates myoblast proliferation and differentiation via the PKA-CREB pathway in Duolang sheep | ||
Cancer Genomics Proteomics Depletion of DNTTIP2 Induces Cell Cycle Arrest in Pancreatic Cancer Cells |
