验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, T-47D cells, HEK-293 cells, MCF-7 cells |
| Positive IF-P detected in | mouse liver tissue |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20057-1-AP targets CDH26 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cadherin-like 26 |
| 别名 | Cadherin-like protein VR20, Cadherin-like protein 26 |
| 计算分子量 | 95 kDa |
| 观测分子量 | 18-22 kDa, 95-100 kDa |
| GenBank蛋白编号 | NM_021810 |
| 基因名称 | CDH26 |
| Gene ID (NCBI) | 60437 |
| RRID | AB_2878637 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IXH8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDH26, also named VR20, is a member of cadherins which are calcium-dependent cell adhesion proteins. Cadherins preferentially interact with themselves in a homophilic manner in connecting cells. Cadherins may thus contribute to the sorting of heterogeneous cell types. CDH26 produces 4 isoforms through alternative splicing, with molecular weights of 13, 18, 92, and 95 kDa, respectively.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDH26 antibody 20057-1-AP | Download protocol |
| IF protocol for CDH26 antibody 20057-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |