验证数据展示
经过测试的应用
Positive WB detected in | mouse kidney tissue, human kidney tissue, HEK-293 cells, rat kidney tissue |
Positive IHC detected in | human kidney tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:16000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15107-1-AP targets Cadherin-16 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | Cadherin-16 fusion protein Ag4343 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | cadherin 16, KSP-cadherin |
别名 | cadherin 16, KSP cadherin, Cadherin-16, CDH16, Kidney specific cadherin, Ksp cadherin |
计算分子量 | 90 kDa |
观测分子量 | 130 kDa |
GenBank蛋白编号 | BC027912 |
基因名称 | Cadherin-16 |
Gene ID (NCBI) | 1014 |
RRID | AB_10596931 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O75309 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cadherin-16 (CDH16), also known as kidney-specific cadherin (Ksp-cadherin), is a tissue-specific member of the cadherin family of adhesion molecules (PMID: 7615566). It is a glycoprotein expressed by tubular epithelial cells in the kidney. Cadherin-16 expression is down-regulated in renal cell carcinoma, suggesting a role in tumor suppression (PMID: 15886705).
实验方案
Product Specific Protocols | |
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WB protocol for Cadherin-16 antibody 15107-1-AP | Download protocol |
IHC protocol for Cadherin-16 antibody 15107-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Int Immunopharmacol Cytoplasmic HMGB1 induces renal tubular ferroptosis after ischemia/reperfusion | ||
Front Cell Dev Biol Transient Receptor Potential Channel 6 Knockout Ameliorates Kidney Fibrosis by Inhibition of Epithelial-Mesenchymal Transition. | ||
J Cancer TMT-labeling Proteomics of Papillary Thyroid Carcinoma Reveal Invasive Biomarkers. | ||
Cell Death Dis Blocking connexin 43 and its promotion of ATP release from renal tubular epithelial cells ameliorates renal fibrosis. |