验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, human kidney tissue, HEK-293 cells, rat kidney tissue |
| Positive IHC detected in | human kidney tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15107-1-AP targets Cadherin-16 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag4343 Product name: Recombinant human CDH16 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 20-350 aa of BC027912 Sequence: AQPAELSVEVPENYGGNFPLYLTKLPLPREGAEGQIVLSGDSGKATEGPFAMDPDSGFLLVTRALDREEQAEYQLQVTLEMQDGHVLWGPQPVLVHVKDENDQVPHFSQAIYRARLSRGTRPGIPFLFLEASDRDEPGTANSDLRFHILSQAPAQPSPDMFQLEPRLGALALSPKGSTSLDHALERTYQLLVQVKDMGDQASGHQATATVEVSIIESTWVSLEPIHLAENLKVLYPHHMAQVHWSGGDVHYHLESHPPGPFEVNAEGNLYVTRELDREAQAEYLLQVRAQNSHGEDYAAPLELHVLVMDENDNVPICPPRDPTVSIPELSP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cadherin 16, KSP-cadherin |
| 别名 | cadherin 16, KSP cadherin, Cadherin-16, CDH16, Kidney specific cadherin, Ksp cadherin |
| 计算分子量 | 90 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC027912 |
| 基因名称 | Cadherin-16 |
| Gene ID (NCBI) | 1014 |
| RRID | AB_10596931 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75309 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cadherin-16 (CDH16), also known as kidney-specific cadherin (Ksp-cadherin), is a tissue-specific member of the cadherin family of adhesion molecules (PMID: 7615566). It is a glycoprotein expressed by tubular epithelial cells in the kidney. Cadherin-16 expression is down-regulated in renal cell carcinoma, suggesting a role in tumor suppression (PMID: 15886705).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Cadherin-16 antibody 15107-1-AP | Download protocol |
| WB protocol for Cadherin-16 antibody 15107-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Cytoplasmic HMGB1 induces renal tubular ferroptosis after ischemia/reperfusion | ||
Front Cell Dev Biol Transient Receptor Potential Channel 6 Knockout Ameliorates Kidney Fibrosis by Inhibition of Epithelial-Mesenchymal Transition. | ||
J Cancer TMT-labeling Proteomics of Papillary Thyroid Carcinoma Reveal Invasive Biomarkers. | ||
Cell Death Dis Blocking connexin 43 and its promotion of ATP release from renal tubular epithelial cells ameliorates renal fibrosis. |
