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验证数据展示

  • WB analysis using 83682-4-RR

    WB analysis using 83682-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83682-4-RR (CDCA7L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 83682-4-RR

    FC experiment of A431 using 83682-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug CDCA7L Recombinant antibody (83682-4-RR, Clone:240639A3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83682-4-RR

    Affinity and Kinetic Characterization of 83682-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83682-4-RR against Human CDCA7L were performed. The affinity constant is 7.75 nM.

CDCA7L Recombinant antibody

CDCA7L Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83682-4-RR

产品说明书

CloneNo. 240639A3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

R1, Protein JPO2, JPO2, HR1, Cell division cycle-associated 7-like protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293T cells, HeLa cells, K-562 cells, MCF-7 cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83682-4-RR targets CDCA7L in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 CDCA7L fusion protein Ag10944 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cell division cycle associated 7-like
别名 R1, Protein JPO2, JPO2, HR1, Cell division cycle-associated 7-like protein
计算分子量 454 aa, 52 kDa
观测分子量52 kDa
GenBank蛋白编号BC009352
基因名称 CDCA7L
Gene ID (NCBI) 55536
RRIDAB_3671283
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96GN5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cell division cycle-associated 7-like protein (CDCA7L) is also known as HR1 and JPO2 and belongs to the JPO protein family. CDCA7L is identified as a target gene of c-Myc and is frequently dysregulated in multiple cancers. CDCA7L can complement the c-Myc transformation-defective mutant W135E and potentiate Myc-mediated transformation (PMID: 24141559, PMID: 37140023). CDCA7L translocates from cytoplasm to nucleus under dexamethasone induction. CDCA7L is widely expressed. CDCA7L has 4 isoforms produced by alternative splicing (refer to UniProt).

实验方案

Product Specific Protocols
WB protocol for CDCA7L antibody 83682-4-RRDownload protocol
FC protocol for CDCA7L antibody 83682-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols