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验证数据展示

  • WB analysis using 83682-4-RR (same clone as 83682-4-PBS)

    WB analysis using 83682-4-RR (same clone as 83682-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83682-4-RR (CDCA7L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83682-4-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83682-4-RR (same clone as 83682-4-PBS)

    FC experiment of A431 using 83682-4-RR (same clone as 83682-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug CDCA7L Recombinant antibody (83682-4-RR, Clone:240639A3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83682-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83682-4-RR

    Affinity and Kinetic Characterization of 83682-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83682-4-RR against Human CDCA7L were performed. The affinity constant is 7.75 nM.

CDCA7L Recombinant monoclonal antibody, PBS Only

CDCA7L Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83682-4-PBS

产品说明书

CloneNo. 240639A3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

R1, Protein JPO2, JPO2, HR1, Cell division cycle-associated 7-like protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CDCA7L Ready-To-Use IHC Kit. CDCA7L primary antibody included.

产品信息

83682-4-PBS targets CDCA7L in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10944

Product name: Recombinant human CDCA7L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-269 aa of BC009352

Sequence: MELATRYQIPKEVADIFNAPSDDEEFVGFRDDVPMETLSSEESCDSFDSLESGKQQDVRFHSKYFTEELRRIFIEDTDSETEDFAGFTQSDLNGKTNPEVMVVESDLSDDGKASLVSEEEEDEEEDKATPRRSRSRRSSIGLRVAFQFPTKKLANKPDKNSSSEQLFSSARLQNEKKTILERKKDCRQVIQREDSTSESEDDSRDESQESSDALLKRTMNIKENKAMLAQLLAELNSMPDFFPVRTPTSASRKKTVRRAFSEGQITRRM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cell division cycle associated 7-like
别名 R1, Protein JPO2, JPO2, HR1, Cell division cycle-associated 7-like protein
计算分子量 454 aa, 52 kDa
GenBank蛋白编号BC009352
基因名称 CDCA7L
Gene ID (NCBI) 55536
RRIDAB_3671283
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ96GN5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cell division cycle-associated 7-like protein (CDCA7L) is also known as HR1 and JPO2 and belongs to the JPO protein family. CDCA7L is identified as a target gene of c-Myc and is frequently dysregulated in multiple cancers. CDCA7L can complement the c-Myc transformation-defective mutant W135E and potentiate Myc-mediated transformation (PMID: 24141559, PMID: 37140023). CDCA7L translocates from cytoplasm to nucleus under dexamethasone induction. CDCA7L is widely expressed. CDCA7L has 4 isoforms produced by alternative splicing (refer to UniProt).

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