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验证数据展示

  • IF Staining of Jurkat using CL594-67145

    IF Staining of Jurkat using CL594-67145

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite®594 CDC25B antibody (CL594-67145, Clone: 1H3A4 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL594-67145

    FC experiment of Jurkat using CL594-67145

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CDC25B (CL594-67145, Clone:1H3A4) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite®594-conjugated CDC25B Monoclonal antibody

CDC25B Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67145

产品说明书

CloneNo. 1H3A4

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

1H3A4, Cdc 25B, CDC25HU2, Cell division cycle 25 homolog B, EC:3.1.3.48

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CDC25B Ready-To-Use IHC Kit. CDC25B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67145-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67145 targets CDC25B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag27193

Product name: Recombinant human CDC25B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 169-398 aa of BC006395

Sequence: SQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSK

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cell division cycle 25 homolog B (S. pombe)
别名 1H3A4, Cdc 25B, CDC25HU2, Cell division cycle 25 homolog B, EC:3.1.3.48
计算分子量 65 kDa
观测分子量 69 kDa
GenBank蛋白编号BC006395
基因名称 CDC25B
Gene ID (NCBI) 994
RRIDAB_3084752
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP30305
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

实验方案

Product Specific Protocols
FC protocol for CL594 CDC25B antibody CL594-67145Download protocol
IF protocol for CL594 CDC25B antibody CL594-67145Download protocol
Standard Protocols
Click here to view our Standard Protocols
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