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验证数据展示

  • IHC staining of human colon cancer using 30451-1-AP (same clone as 30451-1-PBS)

    IHC staining of human colon cancer using 30451-1-AP (same clone as 30451-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30451-1-AP (CDC20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30451-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDC20 antibody included
  • WB analysis using 30451-1-AP (same clone as 30451-1-PBS)

    WB analysis using 30451-1-AP (same clone as 30451-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 30451-1-AP (CDC20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30451-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 30451-1-AP (same clone as 30451-1-PBS)

    IF Staining of U2OS using 30451-1-AP (same clone as 30451-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CDC20 antibody (30451-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30451-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 30451-1-AP (same clone as 30451-1-PBS)

    IF Staining of HEK-293 using 30451-1-AP (same clone as 30451-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CDC20 antibody (30451-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30451-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 30451-1-AP (same clone as 30451-1-PBS)

    IF Staining of HeLa using 30451-1-AP (same clone as 30451-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CDC20 antibody (30451-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30451-1-PBS in a different storage buffer formulation.

CDC20 Polyclonal antibody, PBS Only

CDC20 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30451-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CDC20 Ready-To-Use IHC Kit. CDC20 primary antibody included.

产品信息

30451-1-PBS targets CDC20 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag32712

Product name: Recombinant human CDC20 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-176 aa of BC001088

Sequence: MAQFAFESDLHSLLQLDAPIPNAPPARWQRKAKEAAGPAPSPMRAANRSHSAGRTPGRTPGKSSSKVQTTPSKPGGDRYIPHRSAAQMEVASFLLSKENQPENSQTPTKKEHQKAWALNLNGFDVEEAKILRLSGKPQNAPEGYQNRLKVLYSQKATPGSSRKTCRYIPSLPDRIL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cell division cycle 20 homolog (S. cerevisiae)
别名
计算分子量 55 kDa
观测分子量 51 kDa
GenBank蛋白编号BC001088
基因名称 CDC20
Gene ID (NCBI) 991
RRIDAB_3086319
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12834
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cdc20 (Cell-division cycle protein 20 homologue) is also named as p55CDC and belongs to the WD repeat CDC20/Fizzy family. Cdc20 has important functions in chromosome segregation and mitotic exit. Cdc20 is the target of the spindle assembly checkpoint (SAC) and a key cofactor of the anaphase-promoting complex or cyclosome (APC/CCDC20) E3 ubiquitin ligase, thus regulating APC/C ubiquitin activity on specific substrates for their subsequent degradation by the proteasome (PMID: 28202332). The 55-kDa band corresponded to the size of Cdc20 (PMID: 22566641).

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