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验证数据展示

  • IHC staining of human colon cancer using 30451-1-AP

    IHC staining of human colon cancer using 30451-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30451-1-AP (CDC20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDC20 antibody included
  • WB analysis using 30451-1-AP

    WB analysis using 30451-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 30451-1-AP (CDC20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 30451-1-AP

    IF Staining of U2OS using 30451-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CDC20 antibody (30451-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HEK-293 using 30451-1-AP

    IF Staining of HEK-293 using 30451-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CDC20 antibody (30451-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of HeLa using 30451-1-AP

    IF Staining of HeLa using 30451-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CDC20 antibody (30451-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

CDC20 Polyclonal antibody

CDC20 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30451-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, IF/ICC, ELISA

别名

bA276H19.3, CDC20, CDC20A, p55CDC

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTHP-1 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, HEK-293 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDC20 Ready-To-Use IHC Kit. CDC20 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30451-1-AP targets CDC20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag32712

Product name: Recombinant human CDC20 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-176 aa of BC001088

Sequence: MAQFAFESDLHSLLQLDAPIPNAPPARWQRKAKEAAGPAPSPMRAANRSHSAGRTPGRTPGKSSSKVQTTPSKPGGDRYIPHRSAAQMEVASFLLSKENQPENSQTPTKKEHQKAWALNLNGFDVEEAKILRLSGKPQNAPEGYQNRLKVLYSQKATPGSSRKTCRYIPSLPDRIL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cell division cycle 20 homolog (S. cerevisiae)
别名 bA276H19.3, CDC20, CDC20A, p55CDC
计算分子量 55 kDa
观测分子量 51 kDa
GenBank蛋白编号BC001088
基因名称 CDC20
Gene ID (NCBI) 991
RRIDAB_3086319
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12834
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cdc20 (Cell-division cycle protein 20 homologue) is also named as p55CDC and belongs to the WD repeat CDC20/Fizzy family. Cdc20 has important functions in chromosome segregation and mitotic exit. Cdc20 is the target of the spindle assembly checkpoint (SAC) and a key cofactor of the anaphase-promoting complex or cyclosome (APC/CCDC20) E3 ubiquitin ligase, thus regulating APC/C ubiquitin activity on specific substrates for their subsequent degradation by the proteasome (PMID: 28202332). The 55-kDa band corresponded to the size of Cdc20 (PMID: 22566641).

实验方案

Product Specific Protocols
IF protocol for CDC20 antibody 30451-1-APDownload protocol
IHC protocol for CDC20 antibody 30451-1-APDownload protocol
WB protocol for CDC20 antibody 30451-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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