验证数据展示

WB analysis of HeLa using 19532-1-AP
WB result of CDK1-Specific antibody (19532-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK1-Specific transfected HeLa cells.
WB analysis using 19532-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19532-1-AP (CDK1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 19532-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19532-1-AP (CDK1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 19532-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19532-1-AP (CDK1-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
FC experiment of HeLa using 19532-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDK1-Specific (19532-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
IHC staining of rat liver using 19532-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 19532-1-AP (CDK1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CDK1 antibody included
IHC staining of human cervical cancer using 19532-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19532-1-AP (CDK1-Specific antibody at dilution of 1:200 (under 10x lens).
Ready-to-use IHC kits available with CDK1 antibody included
IHC staining of human cervical cancer using 19532-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19532-1-AP (CDK1-Specific antibody at dilution of 1:200 (under 40x lens).
Ready-to-use IHC kits available with CDK1 antibody included
IF Staining of HeLa using 19532-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK1-Specific antibody (19532-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IHC staining of mouse liver using 19532-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19532-1-AP (CDK1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CDK1 antibody included
IP experiment of HeLa using 19532-1-AP
IP result of anti-CDK1-Specific (IP:19532-1-AP, 4ug; Detection:19532-1-AP 1:5000) with HeLa cells lysate 1600 ug.
IP experiment of HEK-293 using 19532-1-AP
IP result of anti-CDK1-Specific (IP:19532-1-AP, 4ug; Detection:19532-1-AP 1:5000) with HEK-293 cells lysate 1200 ug.

Featured Product
KD/KO验证

CDK1 Polyclonal antibody

CDK1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 19532-1-AP

产品说明书

引用文献(244)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey and More (3)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

别名

CDK1-Specific, CDC2, CDC28A, CDKN1, Cell division protein kinase 1

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 555 CoraLite® Plus 647

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (244)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A431 cells, HeLa cells, HEK-293 cells, COS-7 cells, HepG2 cells, C2C12 cells, C6 cells, Jurkat cells, NIH/3T3 cells, Neuro-2a cells, MCF-7 cells
Positive IP detected in	HeLa cells, HEK-293 cells
Positive IHC detected in	human cervical cancer tissue, mouse liver tissue, rat liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy CDK1 Ready-To-Use IHC Kit. CDK1 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 555 and CoraLite® Plus 647 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 7 publications below
WB	See 222 publications below
IHC	See 24 publications below
IF	See 10 publications below
IP	See 1 publications below
ChIP	See 1 publications below

产品信息

19532-1-AP targets CDK1 in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, ChIP
经测试反应性	human, mouse, rat, monkey
文献引用反应性	human, mouse, rat, pig, chicken, sheep
免疫原	Peptide 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	cell division cycle 2, G1 to S and G2 to M
别名	CDK1-Specific, CDC2, CDC28A, CDKN1, Cell division protein kinase 1
计算分子量	34 kDa
观测分子量	30-34 kDa
GenBank蛋白编号	NM_001786
基因名称	CDK1
Gene ID (NCBI)	983
RRID	AB_10638617
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P06493
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa. This antibody may have cross reaction with CDK2.

实验方案

Product Specific Protocols
IF protocol for CDK1 antibody 19532-1-AP	Download protocol
IHC protocol for CDK1 antibody 19532-1-AP	Download protocol
IP protocol for CDK1 antibody 19532-1-AP	Download protocol
WB protocol for CDK1 antibody 19532-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Signal Transduct Target Ther SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis Authors - Saisai Guo KD Validated View Article
human	WB	Nat Cell Biol Caspase-2 is a condensate-mediated deubiquitinase in protein quality control Authors - Yingwei Ge View Article
human	IHC	Cell Res Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma. Authors - Junya Peng View Article
human	WB	Nat Struct Mol Biol Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis Authors - Wei Zhang View Article
human	WB	Nat Commun Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells Authors - Qiuhong Zhu View Article
mouse	WB	Nat Commun Foxk1 and Foxk2 promote cardiomyocyte proliferation and heart regeneration Authors - Dongcheng Cai View Article

验证数据展示

WB analysis of HeLa using 19532-1-AP

WB analysis using 19532-1-AP

WB analysis using 19532-1-AP

WB analysis using 19532-1-AP

FC experiment of HeLa using 19532-1-AP

IHC staining of rat liver using 19532-1-AP

IHC staining of human cervical cancer using 19532-1-AP

IHC staining of human cervical cancer using 19532-1-AP

IF Staining of HeLa using 19532-1-AP

IHC staining of mouse liver using 19532-1-AP

IP experiment of HeLa using 19532-1-AP

IP experiment of HEK-293 using 19532-1-AP

CDK1 Polyclonal antibody

CDK1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis

Caspase-2 is a condensate-mediated deubiquitinase in protein quality control

Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma.

Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis

Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells

Foxk1 and Foxk2 promote cardiomyocyte proliferation and heart regeneration