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验证数据展示

  • WB analysis using 14307-1-AP

    WB analysis using 14307-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 14307-1-AP

    IHC staining of human cervical cancer using 14307-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human cervical cancer using 14307-1-AP

    IHC staining of human cervical cancer using 14307-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 14307-1-AP

    IF Staining of HeLa using 14307-1-AP

    Immunofluorescent analysis of Hela cells, using CDC16 antibody 14307-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

CDC16/APC6 Polyclonal antibody

CDC16/APC6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14307-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

CDC16, APC6, CDC16Hs, CDC16 homolog, Anaphase-promoting complex subunit 6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, NIH/3T3 cells, Jurakt cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14307-1-AP targets CDC16/APC6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag5427

Product name: Recombinant human CDC16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 178-527 aa of BC010875

Sequence: IGTLVELNKANELFYLSHKLVDLYPSNPVSWFAVGCYYLMVGHKNEHARRYLSKATTLEKTYGPAWIAYGHSFAVESEHDQAMAAYFTAAQLMKGCHLPMLYIGLEYGLTNNSKLAERFFSQALSIAPEDPFVMHEVGVVAFQNGEWKTAEKWFLDALEKIKAIGNEVTVDKWEPLLNNLGHVCRKLKKYAEALDYHRQALVLIPQNASTYSAIGYIHSLMGNFENAVDYFHTALGLRRDDTFSVTMLGHCIEMYIGDSEAYIGADIKDKLKCYDFDVHTMKTLKNIISPPWDFREFEVEKQTAEETGLTPLETSRKTPDSRPSLEETFEIEMNESDMMLETSMSDHST

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cell division cycle 16 homolog (S. cerevisiae)
别名 CDC16, APC6, CDC16Hs, CDC16 homolog, Anaphase-promoting complex subunit 6
计算分子量 72 kDa
观测分子量 72 kDa
GenBank蛋白编号BC010875
基因名称 CDC16/APC6
Gene ID (NCBI) 8881
RRIDAB_2074638
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13042
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CDC16/APC6 antibody 14307-1-APDownload protocol
IHC protocol for CDC16/APC6 antibody 14307-1-APDownload protocol
WB protocol for CDC16/APC6 antibody 14307-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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