验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28178-1-AP targets CDC123/C10orf7 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | CDC123/C10orf7 fusion protein Ag26828 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cell division cycle 123 homolog (S. cerevisiae) |
| 别名 | CDC123, C10orf7, Protein D123, HT-1080, HT 1080 |
| 计算分子量 | 39 kDa |
| 观测分子量 | 35-39 kDa |
| GenBank蛋白编号 | BC001600 |
| 基因名称 | CDC123 |
| Gene ID (NCBI) | 8872 |
| RRID | AB_2881081 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75794 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDC123/C10orf7 antibody 28178-1-AP | Download protocol |
| IHC protocol for CDC123/C10orf7 antibody 28178-1-AP | Download protocol |
| IF protocol for CDC123/C10orf7 antibody 28178-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |