CoraLite® Plus 488-conjugated CD99 Recombinant antibody

CD99 Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-84580-4

产品说明书

CloneNo. 241737H1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

12E7, 241737H1, CD99 antigen, CD99 molecule, E2 antigen

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存


经过测试的应用

Positive IF/ICC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 84580-4-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84580-4 targets CD99 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD99 molecule
别名 12E7, 241737H1, CD99 antigen, CD99 molecule, E2 antigen
计算分子量 19 kDa, 16 kDa, 17 kDa
观测分子量28 kDa, 32 kDa
GenBank蛋白编号BC021620
基因名称 CD99
Gene ID (NCBI) 4267
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP14209
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD99 antibody CL488-84580-4Download protocol
Standard Protocols
Click here to view our Standard Protocols
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