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验证数据展示

  • IF Staining of Jurkat using 66972-1-Ig (same clone as 66972-1-PBS)

    IF Staining of Jurkat using 66972-1-Ig (same clone as 66972-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD97 antibody (66972-1-Ig, Clone: 3F10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66972-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 66972-1-Ig (same clone as 66972-1-PBS)

    WB analysis of K-562 using 66972-1-Ig (same clone as 66972-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66972-1-PBS in a different storage buffer formulation.

CD97 Monoclonal antibody, PBS Only

CD97 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66972-1-PBS

产品说明书

CloneNo. 3F10C2

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

ADGRE5, Adhesion G protein-coupled receptor E5, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5 subunit beta, CD97 antigen

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66972-1-PBS targets CD97 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag27991

Product name: Recombinant human CD97 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 21-350 aa of BC026690

Sequence: QDSRGCARWCPQNSSCVNATACRCNPGFSSFSEIITTPTETCDDINECATPSKVSCGKFSDCWNTEGSYDCVCSPGYEPVSGAKTFKNESENTCQDVDECSSGQHQCDSSTVCFNTVGSYSCRCRPGWKPRHGIPNNQKDTVCEDMTFSTWTPPPGVHSQTLSRFFDKVQDLGRDSKTSSAEVTIQNVIKLVDELMEAPGDVEALAPPVRHLIATQLLSNLEDIMRILAKSLPKGPFTYISPSNTELTLMIQERGDKNVTMGQSSARMKLNWAVAAGAEDPGPAVAGILSIQNMTTLLANASLNLHSKKQAELEEIYESSIRGVQLRRLS

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 CD97 molecule
别名 ADGRE5, Adhesion G protein-coupled receptor E5, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5 subunit beta, CD97 antigen
计算分子量 722 aa, 79 kDa
观测分子量 75 kDa
GenBank蛋白编号BC026690
基因名称 CD97
Gene ID (NCBI) 976
RRIDAB_2882293
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP48960
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).

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