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验证数据展示

  • WB analysis using 84832-1-RR

    WB analysis using 84832-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84832-1-RR (CD90.1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 84832-1-RR

    IHC staining of mouse spleen using 84832-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84832-1-RR (CD90.1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 84832-1-RR

    IHC staining of mouse spleen using 84832-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84832-1-RR (CD90.1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 84832-1-RR

    IF Staining of mouse spleen using 84832-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD90.1 antibody (84832-1-RR, Clone: 241542D7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84832-1-RR

    Affinity and Kinetic Characterization of 84832-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84832-1-RR against Mouse CD90.1 were performed. The affinity constant is 17.0 nM.

CD90.1 Recombinant antibody

CD90.1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84832-1-RR

产品说明书

CloneNo. 241542D7

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF-P, ELISA

别名

CD90, Thy1, Thy 1, 241542D7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inFVB mouse brain tissue, FVB mouse spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84832-1-RR targets CD90.1 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 thymus cell antigen 1, theta
别名 CD90, Thy1, Thy 1, 241542D7
观测分子量25 kDa
基因名称 CD90
Gene ID (NCBI) 21838
RRIDAB_3672141
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR and FVB (PMID: 5919593).

实验方案

Product Specific Protocols
WB protocol for CD90.1 antibody 84832-1-RRDownload protocol
IHC protocol for CD90.1 antibody 84832-1-RRDownload protocol
IF protocol for CD90.1 antibody 84832-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols