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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84832-1-RR (same clone as 84832-1-PBS)

    WB analysis using 84832-1-RR (same clone as 84832-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84832-1-RR (CD90.1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84832-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 84832-1-RR (same clone as 84832-1-PBS)

    IHC staining of mouse spleen using 84832-1-RR (same clone as 84832-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84832-1-RR (CD90.1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84832-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 84832-1-RR (same clone as 84832-1-PBS)

    IHC staining of mouse spleen using 84832-1-RR (same clone as 84832-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84832-1-RR (CD90.1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84832-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 84832-1-RR (same clone as 84832-1-PBS)

    IF Staining of mouse spleen using 84832-1-RR (same clone as 84832-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD90.1 antibody (84832-1-RR, Clone: 241542D7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84832-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84832-1-RR

    Affinity and Kinetic Characterization of 84832-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84832-1-RR against Mouse CD90.1 were performed. The affinity constant is 17.0 nM.

Mouse CD90.1 Recombinant antibody, PBS Only

CD90.1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 84832-1-PBS

产品说明书

CloneNo. 241542D7

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

CD90, Thy1, Thy 1, 241542D7

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84832-1-PBS targets CD90.1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 thymus cell antigen 1, theta
别名 CD90, Thy1, Thy 1, 241542D7
观测分子量25 kDa
基因名称 CD90
Gene ID (NCBI) 21838
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR and FVB (PMID: 5919593).